製品の概要

  • 製品名
    Anti-IL10 antibody [B-S10]
    IL10 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [B-S10] to IL10
  • 特異性
    This antibody recognizes both natural and recombinant IL10.
  • アプリケーション
    適用あり: Blocking, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant human IL10.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Ion Exchange Chromatography
  • 特記事項(精製)
    This antibody is sterile filtered through 0.22 µm and treated to remove endotoxins. It is carrier and preservative free.
  • ポリ/モノ
    モノクローナル
  • クローン名
    B-S10
  • ミエローマ
    x63-Ag8.653
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab47222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Blocking
ELISA
  • 追加情報
    BL: Use at an assay dependent dilution.
    The antibody blocks IL10 induced proliferation on MC/9 cell line.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
    • 組織特異性
      Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
    • 配列類似性
      Belongs to the IL-10 family.
    • 細胞内局在
      Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CSIF antibody
      • Cytokine synthesis inhibitory factor antibody
      • GVHDS antibody
      • IL 10 antibody
      • IL-10 antibody
      • IL10 antibody
      • IL10_HUMAN antibody
      • IL10A antibody
      • Interleukin 10 antibody
      • Interleukin-10 antibody
      • MGC126450 antibody
      • MGC126451 antibody
      • T-cell growth inhibitory factor antibody
      • TGIF antibody
      see all

    画像

    • We examined the effect of anti IL-10 to study the regulatory role of IL-10 and the interaction of IL-10 with its receptor. Measurement of neutralizing activity of anti IL-10 by TNFa-ELISA. IL-10 decreased the LPS-induced production of TNFa by Monocytes and recombinant IL-10 is neutralized by increasing concentrations of anti IL-10 antibody. The TNFa production showed a dependence on antibody concentration of anti IL-10.
      ab47222 used at a 1/2000 dilution.

      See Abreview

    参考文献

    ab47222 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    ELISA
    Type
    Direct
    Sample
    Human Cell (Monocytes)
    Specification
    Monocytes
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Username

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    投稿 Feb 13 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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