製品の概要

  • 製品名Anti-IL10 antibody
    IL10 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IL10
  • 特異性Direct ELISA results in no cross reactivity against cytokines other than IL10. The antiserum detects recombinant and native IL-10 present in body fluids and cell supernatants in various assays (e.g. IL-1 stimulated IL-10 production from fibroblasts).
  • アプリケーション適用あり: WB, IHC-Fr, IP, ELISA, Neutralising, RIA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant (Human) full length protein produced in E. coli

  • ポジティブ・コントロール
    • WB: 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC)(PBMC stimulated for 24 hours with 1% (v/v) human serum plus 10 ng/mL E.coli LPS); IL-1 stimulated IL- 10 production from fibroblasts, recombinant protein

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab34843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/400 - 1/800. Predicted molecular weight: 21 kDa. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule.
IHC-Fr 1/400.
IP 1/400 - 1/800. Pre-clearing with a non-specific rabbit IgG is recommended to reduce background.
ELISA 1/400 - 1/1200. (as detection antibody, using a monoclonal antibody for capture).
Neutralising 1/400. Incubate the samples with the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended.
RIA 1/4000.
IHC-P 1/400.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • 組織特異性Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • 配列類似性Belongs to the IL-10 family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CSIF antibody
    • Cytokine synthesis inhibitory factor antibody
    • GVHDS antibody
    • IL 10 antibody
    • IL-10 antibody
    • IL10 antibody
    • IL10_HUMAN antibody
    • IL10A antibody
    • Interleukin 10 antibody
    • Interleukin-10 antibody
    • MGC126450 antibody
    • MGC126451 antibody
    • T-cell growth inhibitory factor antibody
    • TGIF antibody
    see all

Anti-IL10 antibody 画像

  • ab34843 staining IL10 in Human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 20 minutes at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/200 in 1% BSA in PBS) for 16 hours at 4°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

  • Immunofluorescence analysis of Human peripheral blood mononuclear cells, staining IL10 with ab34843.

    Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS and blocked with 1% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 1% BSA in PBS) for 24 hours at 4°C. An AlexaFluor®594-conjugated goat anti-rabbit polyclonal IgG (1/250) was used as the secondary antibody.

    See Abreview

Anti-IL10 antibody (ab34843) 使用論文

This product has been referenced in:
  • Kaewkangsadan V  et al. Crucial Contributions by T Lymphocytes (Effector, Regulatory, and Checkpoint Inhibitor) and Cytokines (TH1, TH2, and TH17) to a Pathological Complete Response Induced by Neoadjuvant Chemotherapy in Women with Breast Cancer. J Immunol Res 2016:4757405 (2016). IHC-P ; Human . Read more (PubMed: 27777963) »
  • Furukawa S  et al. Preferential M2 macrophages contribute to fibrosis in IgG4-related dacryoadenitis and sialoadenitis, so-called Mikulicz's disease. Clin Immunol 156:9-18 (2015). IHC-P ; Human . Read more (PubMed: 25450336) »

See all 11 Publications for this product

Product Wall

Application Western blot
Sample Cynomolgus Monkey Tissue lysate - whole (retina)
Gel Running Conditions Reduced Denaturing (4-15% gel)
Loading amount 50 µg
Specification retina
Blocking step Milk as blocking agent for 1 hour(s) and 45 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Nov 18 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cynomolgus Monkey Tissue sections (Retina)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Specification Retina
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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投稿 Nov 02 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human head & neck squamous cell carcinoma)
Specification Human head & neck squamous cell carcinoma
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100 (0.1%) for 5 min
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
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投稿 Jan 29 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human peripheral blood mononuclear cells)
Specification Human peripheral blood mononuclear cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100 in PBS, 3min
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
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投稿 Jan 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colon)
Specification Colon
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Diva Decloaker
Permeabilization No
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
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投稿 Sep 10 2012

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"