アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。
Recombinant (Human) full length protein produced in E. coli
Our Abpromise guarantee covers the use of ab34843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/400 - 1/800. Predicted molecular weight: 21 kDa. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule.|
|IP||1/400 - 1/800. Pre-clearing with a non-specific rabbit IgG is recommended to reduce background.|
|ELISA||1/400 - 1/1200. (as detection antibody, using a monoclonal antibody for capture).|
|Neutralising||1/400. Incubate the samples with the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended.|
|ICC/IF||Use at an assay dependent concentration.|