Anti-IL1 Receptor I 抗体 [40101] (ab115497)

製品の概要

  • 製品名
    Anti-IL1 Receptor I antibody [40101]
    IL1 Receptor I 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [40101] to IL1 Receptor I
  • 特異性
    ab115497 reacts with cell surface and soluble human IL1 Receptor I. The specific epitope is unknown. ab115497 does not block binding of IL-1 to the receptor, and does not cross-react with receptors for human IL-2 or IL-4.
  • アプリケーション
    適用あり: ICC, ELISA, IHC-P, RIAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment corresponding to the extracellular domain of Human IL1 Receptor I.

  • ポジティブ・コントロール
    • Human Placenta tissue.
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab115497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use a concentration of 10 - 100 µg/ml.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
RIA Use at an assay dependent concentration. Concentrations of 20-30 ng/ml will bind radiolabeled IL1 Receptor I in a radioimmunoassay format.

ターゲット情報

  • 機能
    Receptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1RA). Binding to the agonist leads to the activation of NF-kappa-B. Signaling involves formation of a ternary complex containing IL1RAP, TOLLIP, MYD88, and IRAK1 or IRAK2.
  • 配列類似性
    Belongs to the interleukin-1 receptor family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 TIR domain.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Antigen CD121a antibody
    • CD121 antigen-like family member A antibody
    • CD121a antibody
    • CD121a antigen antibody
    • D2S1473 antibody
    • IL 1 antibody
    • IL 1R 1 antibody
    • IL 1R alpha antibody
    • IL 1RT1 antibody
    • IL-1R-1 antibody
    • IL-1R-alpha antibody
    • IL-1RT-1 antibody
    • IL-1RT1 antibody
    • IL1 inhibitor antibody
    • IL1R antibody
    • IL1R1 antibody
    • IL1R1_HUMAN antibody
    • IL1RA antibody
    • IL1RN antibody
    • IL1RT1 antibody
    • Interleukin 1 receptor alpha type 1 antibody
    • Interleukin 1 receptor antagonist protein (IRAP) antibody
    • Interleukin 1 receptor type I antibody
    • Interleukin receptor 1 antibody
    • Interleukin-1 receptor alpha antibody
    • Interleukin-1 receptor type 1 antibody
    • Interleukin-1 receptor type I antibody
    • p80 antibody
    see all

画像

  • ab115497, at 10 µg/ml, staining IL1 Receptor I in formalin-fixed, paraffin-embedded Human Placenta tissue by Immunohistochemistry using a biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

参考文献

ab115497 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
Sample
Mouse Tissue sections (brain)
Specification
brain
Permeabilization
No
Fixative
Paraformaldehyde
Username

Ms. Michaela Blanfeld

Verified customer

投稿 Nov 18 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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