製品の概要

  • 製品名Anti-IL1 beta antibody (HRP)
    IL1 beta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IL1 beta (HRP)
  • 標識HRP
  • 特異性ab106035 is primarily directed against mature, 17,000 MW Mouse IL1 beta. It will recognize 10% of the non-denatured (native) precursor 31,000 MW Mouse IL1 beta containing samples but will primarily detect all of the 17,000 MW mature molecule. ab106035 does not detect IL1 alpha.
  • アプリケーション適用あり: WB, IHC-P, Flow Cyt, IP, RIA, IHC-Fr, ELISA, Neutralisingmore details
  • 種交差性
    交差種: Mouse, Rat
    非交差種: Human, Non Human Primates
  • 免疫原

    Recombinant Mouse IL1 beta produced in E.coli. The MW of recombinant mouse IL1 beta was 17 kDa

  • ポジティブ・コントロール
    • Rat and Mouse endotoxin stimulated peripheral blood mononuclear cells (PBMC).
  • 特記事項Do NOT add Sodium Azide!

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab106035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Predicted molecular weight: 17, 30 kDa.
IHC-P 1/500 - 1/2500.
Flow Cyt Use at an assay dependent concentration. Caution should be exhibited as the F(c) domain of the Rabbit IgG molecule may interact with cells non-specifically.
IP Use at an assay dependent concentration. Preclearing the preparation with a non-specific Rabbit IgG to reduce background is suggested.
RIA Use at an assay dependent concentration.
IHC-Fr 1/500 - 1/2500.
ELISA 1/10000 - 1/50000. Best used as the second antibody in combination with a monoclonal antibody as a capture antibody.
Neutralising Use at an assay dependent concentration. It is recommended to incubate the sample with a dilution of the antibody for at least 4 hours before being tested.

ターゲット情報

  • 機能Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.
  • 組織特異性Expressed in activated monocytes/macrophages (at protein level).
  • 配列類似性Belongs to the IL-1 family.
  • 翻訳後修飾Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.
  • 細胞内局在Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Catabolin antibody
    • H1 antibody
    • IL 1 antibody
    • IL 1 beta antibody
    • IL-1 beta antibody
    • IL1 BETA antibody
    • IL1B antibody
    • IL1B_HUMAN antibody
    • IL1F2 antibody
    • Interleukin 1 beta antibody
    • Interleukin-1 beta antibody
    • OAF antibody
    • OTTHUMP00000162031 antibody
    • Preinterleukin 1 beta antibody
    • Pro interleukin 1 beta antibody
    see all

Anti-IL1 beta antibody (HRP) (ab106035) 使用論文

This product has been referenced in:
  • Tang YL  et al. TLR4/NF-?B signaling contributes to chronic unpredictable mild stress-induced atherosclerosis in ApoE-/- mice. PLoS One 10:e0123685 (2015). WB ; Mouse . Read more (PubMed: 25860573) »

See 1 Publication for this product

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Thank you for submitting an Abreview of ab106035. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in a tested species and application, we would like to follow up ...

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Application Western blot
Sample Mouse Cell lysate - whole cell (macrophgaes cell line)
Loading amount 50 µg
Specification macrophgaes cell line
Treatment LPS
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Varkha Agrawal

Verified customer

投稿 Sep 21 2012

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The anti-IL1 beta antibody (HRP) (ab106035) was raised against the full length mouse IL1 beta protein expressed in E. coli. This antibody has been validated in ELISA and would therefore be guaranteed by the Abprom...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"