製品の概要

  • 製品名Anti-IL1 beta antibody
    IL1 beta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IL1 beta
  • 特異性Primarily directed against mature 17kDa human IL-1beta. In ELISA formats and other immunoreactive assays, the antibody will recognize 10% of the non-denatured (native) 31kDa IL-1beta precursor containing samples, but will detect all of the 17kDa mature molecule. Also detects primate IL-1beta in the same formats using similar dilutions. The antiserum does not recognize human IL-1alpha.
  • アプリケーション適用あり: WB, IP, ELISA, IHC-P, RIA, IHC-Fr, Functional Studies, Flow Cyt, Neutralising, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Non Human Primates
  • 免疫原

    Recombinant 153 aa human IL-1beta produced in E.coli. MW of recombinant IL-1beta 17kDa, with the N-terminal amino acid at position alanine 117. This is the cleavage site generated by the IL-1beta converting enzyme (ICE, capase-1).

  • 特記事項Immunohistochemistry 1/100 to 1/200 (paraffin or cryofixation can be used; 1/100 for staining intracellular IL-1beta). ELISA 1/200 to 1/1000 (this antibody is best used as the second antibody with a monoclonal as a capture antibody). Radio immunoassay 1/8000 Neutralization of IL-1beta activity in bioassays 1/100 ( >4 hours incubation, normal rabbit IgG as negative control) FACS analysis - caution, F(c) domain of rabbit IgG may interact with cells non-specifically.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration.

1/1000 - 1/2000 (antigen - supernatants or lysates of 2 x 106 endotoxin-stimulated human PBMC. Denatured 31kDa precursor IL-1beta will be recognized, but often migrates as a 35 kDa band).

IP Use at an assay dependent concentration. 1/400 - 1/800 (pre-clearing with a non-specific rabbit IgG is helpful to reduce background).
ELISA 1/500 - 1/2000.
IHC-P 1/100 - 1/200.
RIA 1/8000.
IHC-Fr 1/100 - 1/200.
Functional Studies Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

F(c) domain of rabbit IgG may interact with cells non-specifically!

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Neutralising 1/100. Neutralization of IL-1beta activity in bioassays 1/100 ( >4 hours incubation, normal rabbit IgG as negative control). ab2105 does not neutralize the biological activity of murine, rat or rabbit IL1 beta.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.
  • 組織特異性Expressed in activated monocytes/macrophages (at protein level).
  • 配列類似性Belongs to the IL-1 family.
  • 翻訳後修飾Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.
  • 細胞内局在Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Catabolin antibody
    • H1 antibody
    • IL 1 antibody
    • IL 1 beta antibody
    • IL-1 beta antibody
    • IL1 BETA antibody
    • IL1B antibody
    • IL1B_HUMAN antibody
    • IL1F2 antibody
    • Interleukin 1 beta antibody
    • Interleukin-1 beta antibody
    • OAF antibody
    • OTTHUMP00000162031 antibody
    • Preinterleukin 1 beta antibody
    • Pro interleukin 1 beta antibody
    see all

Anti-IL1 beta antibody 画像

  • All lanes : Anti-IL1 beta antibody (ab2105) at 1/1000 dilution

    Lane 1 : Human peripheral blood mononuclear cell lysate - PBMC's unstimulated
    Lane 2 : Human peripheral blood mononuclear cell lysate - monocytes unstimulated
    Lane 3 : Human peripheral blood mononuclear cell lysate - monocytes stimulated with LPS
    Lane 4 : Human peripheral blood mononuclear cell lysate - positive control (rh IL-1 beta protein)

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/2000 dilution

    Performed under reducing conditions.

    Observed band size : 35 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 minutes

    This image is courtesy of an anonymous Abreview

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  • ab2105 at 1/20 staining human liver tissue sections by IHC-P. The cells were paraformaldehyde fixed and a heat mediated antigen retrieval step was performed. The tissue was incubated with the tissue overnight at room temperature. A biotinylated donkey anti-rabbit IgG was used as the secondary antibody and this was detected using streptavidin HRP.

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  • ab2105 staining IL1 beta in human keratinocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed in methanol, permeabilized in 0,1% Saponin / PBS and then blocked using 4% BSA for 30 minutes at 25°C. Samples were then incubated with ab2105 at a 1/100 dilution for 2 hours at 4°C. An Alexa-fluor 488 conjugated goat anti-rabbit polyclonal was used as the secondary antibody at a 1/100 dilution.

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  • ab2105 staining IL1 beta in Human monocytes by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with 0.1% saponin in PBS. The sample was incubated with the primary antibody (1/500 in 0.1% saponin + 1% FCS in PBS) for 45 minutes at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG (1/100) was used as the secondary antibody.

    1 - unstimulated monocytes. 2 - monocytes stimulated with LPS.

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  • Lane 1 :
    Lanes 2 - 5 : Anti-IL1 beta antibody (ab2105) at 1/1000 dilution

    Lane 1 : Ladder (top to bottom: p35, p25, p20, p17, p11)
    Lane 2 : Human THP-1 leukemic monocyte cell lysate, no LPS at 20 µg
    Lane 3 : Human THP-1 leukemic monocyte supernatant, no LPS at 20 µg
    Lane 4 : Human THP-1 leukemic monocyte cell lysate, LPS stimulation at 20 µg
    Lane 5 : Human THP-1 leukemic monocyte supernatant, LPS stimulation at 20 µg

    Secondary
    Lanes 2 - 5 : IRDye® 800CW-conjugated Donkey anti-rabbit IgG monoclonal at 1/5000 dilution


    Performed under reducing conditions.

    Observed band size : 17,30 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

    Both mature and pro-IL-1beta bands, p17 and p31 were detected by ab2105 in supernatant from THP-1 cells stimulated with LPS. Only pro-IL-1beta band (p31) was detected in supernatant from THP-1 cells not stimulated with LPS.

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  • ab2105 staining IL1 beta in rat alveolar macrophage tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Tissue was fixed with paraformaldehyde and blocked with blocking agent for 30 minutes at room temperature; antigen retrieval was by heat mediation with a citrate buffer. Samples were incubated with primary antibody (1/100 in diluent) for 2 hours. An HRP-conjugated rabbit monoclonal IgG (1/500) was used as the secondary antibody.

    See Abreview

Anti-IL1 beta antibody (ab2105) 使用論文

This product has been referenced in:
  • Ashok A  et al. Exposure to As-, Cd-, and Pb-mixture induces Aß, amyloidogenic APP processing and cognitive impairments via oxidative stress-dependent neuroinflammation in young rats. Toxicol Sci 143:64-80 (2015). Read more (PubMed: 25288670) »
  • Zhang J  et al. Effects of p75 neurotrophin receptor on regulating hypoxia-induced angiogenic factors in retinal pigment epithelial cells. Mol Cell Biochem 398:123-34 (2015). WB ; Human . Read more (PubMed: 25200140) »

See all 19 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Colon)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Target Retrieval Solution, ph6.0
Permeabilization No
Specification Colon
Fixative Formaldehyde
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投稿 Aug 19 2016

Application Western blot
Sample Cynomolgus Monkey Tissue lysate - whole (Macaque Retina Lysate)
Gel Running Conditions Reduced Denaturing (4-15%)
Loading amount 200 µg
Specification Macaque Retina Lysate
Blocking step Milk as blocking agent for 2 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Aug 15 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Muller Cell Line MIO-M1)
Permeabilization Yes - 0.1% TritonX-100
Specification Muller Cell Line MIO-M1
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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投稿 Aug 01 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cynomolgus Monkey Tissue sections (Macaque retina)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH 6
Permeabilization No
Specification Macaque retina
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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投稿 Mar 10 2016

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Sample Human Tissue sections (lung)
Specification lung
Permeabilization Yes - 0,1% Saponin / PBS
Fixative Methanol
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投稿 Oct 14 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6,0
Sample Human Tissue sections (skin)
Specification skin
Permeabilization Yes - 0,1% Saponin/PBS
Fixative Paraformaldehyde
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投稿 Oct 11 2013

Application ELISA
Sample Human Cell (supernatens from macrophages)
Specification supernatens from macrophages
Type Sandwich (Capture)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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投稿 Oct 08 2013

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization Yes - 0,1% Saponin / PBS
Sample Human Cell (human monocytes)
Specification human monocytes
Gating Strategy no gate
Preparation Cell harvesting/tissue preparation method: isolated by gradient centrifugation and followed by magnetic isolation
Sample buffer: 0,1% Saponin /1% FCS/ PBS
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投稿 Oct 08 2013

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (5-16% gel)
Sample Human Cell lysate - whole cell (peripheral blood mononuclear cells)
Specification peripheral blood mononuclear cells
Treatment see below
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Oct 07 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Human Cell lysate - whole cell (THP-1 human leukemic monocyte)
Specification THP-1 human leukemic monocyte
Treatment 100 nM PMA for 24 hrs, 100 ng/ml LPS for 4 hrs
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C
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投稿 Aug 12 2013

1-10 of 36 Abreviews or Q&A

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