製品の概要

  • 製品名Anti-IL1 beta antibody [8516.311]
    IL1 beta 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [8516.311] to IL1 beta
  • 特異性By ELISA and immunoblotting, the antibody shows < 5% cross-reactivity with rhIL1 alpha, rmIL1 beta. In addition the antibody shows no cross-reactivity with rmIl1 alpha, rhIL1 ralpha, rmIL1 ralpha, and rrIL1 ralpha. The antibody will not neutralize the biological activity of rhIL1 alpha, rmIL1 alpha or rmIL1 beta.
  • アプリケーション適用あり: ELISA, WB, Neutralisingmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant human IL1 beta (rhIL1 beta), expressed in E. coli.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 31 kDa.
Neutralising Use at an assay dependent dilution.

ターゲット情報

  • 機能Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.
  • 組織特異性Expressed in activated monocytes/macrophages (at protein level).
  • 配列類似性Belongs to the IL-1 family.
  • 翻訳後修飾Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.
  • 細胞内局在Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Catabolin antibody
    • H1 antibody
    • IL 1 antibody
    • IL 1 beta antibody
    • IL-1 beta antibody
    • IL1 BETA antibody
    • IL1B antibody
    • IL1B_HUMAN antibody
    • IL1F2 antibody
    • Interleukin 1 beta antibody
    • Interleukin-1 beta antibody
    • OAF antibody
    • OTTHUMP00000162031 antibody
    • Preinterleukin 1 beta antibody
    • Pro interleukin 1 beta antibody
    see all

Anti-IL1 beta antibody [8516.311] (ab10749) 使用論文

This product has been referenced in:
  • Lucchesi W  et al. Differential gene regulation by Epstein-Barr virus type 1 and type 2 EBNA2. J Virol 82:7456-66 (2008). WB, ELISA ; Human . Read more (PubMed: 18480445) »
  • Pioli PA  et al. Lipopolysaccharide-induced IL-1 beta production by human uterine macrophages up-regulates uterine epithelial cell expression of human beta-defensin 2. J Immunol 176:6647-55 (2006). ELISA ; Human . Read more (PubMed: 16709823) »

See all 2 Publications for this product

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