The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報 ELISA: Use at a concentration of 0.15 - 0.3 µg/ml. Used in conjunction with compatible secondary reagents, this allows the detection of at least 0.2 ng/well of recombinant Rat IL1 alpha.
WB: Use at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL1 alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Predicted molecular weight: 31 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
配列類似性Belongs to the IL-1 family.
ドメインThe similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
細胞内局在Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.