製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab69930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
ELISA 1/10000.

ターゲット情報

  • 機能Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner.
  • 組織特異性Expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus.
  • 配列類似性Belongs to the Ikaros C2H2-type zinc-finger protein family.
    Contains 6 C2H2-type zinc fingers.
  • 翻訳後修飾Phosphorylation on tyrosine residues induced by IL2 is required for dissociation from HRAS and nuclear translocation of IKZF3 in T-cells. Phosphorylation on tyrosine residues induced by IL4 is required for dissociation from Bcl-X(L) in T-cells.
  • 細胞内局在Nucleus. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AIO antibody
    • Aiolos antibody
    • IKAROS family zinc finger 3 (Aiolos) antibody
    • IKAROS family zinc finger 3 antibody
    • Ikaros family zinc finger protein 3 antibody
    • IKZF 3 antibody
    • IKZF3 antibody
    • IKZF3_HUMAN antibody
    • zinc finger DNA binding protein Aiolos antibody
    • Zinc finger protein Aiolos antibody
    • Zinc finger protein subfamily 1A 3 (Aiolos) antibody
    • Zinc finger protein subfamily 1A 3 antibody
    • Zinc finger protein subfamily 1A, member 3 antibody
    • ZNFN1A3 antibody
    see all

Anti-IKZF3 antibody 画像

  • All lanes : Anti-IKZF3 antibody (ab69930) at 1/500 dilution

    Lane 1 : COLO cell extract
    Lane 2 : COLO cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 58 kDa
    Observed band size : 58 kDa
  • ICC/IF image of ab69930 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69930, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-IKZF3 antibody (ab69930) 使用論文

This product has been referenced in:

See 1 Publication for this product

Product Wall

I now have the information from the source of the Anti-IKZF3 antibody (ab56867). Unfortunately, epitope mapping has as yet not been performed with this antibody. We cannot therefore predict which isoforms (apart from isoform 1 which the antibody was ra...

Read More

Thank you for contacting us and your interest in our products.

In regards to the Anti-IKZF3 antibody (ab56867), the antibody is raised against the full lenght recombinantly expressed human protein. From performing sequence alignment, which is...

Read More

Thank you for contacting Abcam regarding our IKZF3 antibodies.


I have contacted the laboratory and unfortunately, a blocking peptide is not available for this antibody. I do apologize for any inconvenience this may cause. Please do not ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"