製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab64400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 58 kDa).
IP Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner.
  • 組織特異性
    Expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus.
  • 配列類似性
    Belongs to the Ikaros C2H2-type zinc-finger protein family.
    Contains 6 C2H2-type zinc fingers.
  • 翻訳後修飾
    Phosphorylation on tyrosine residues induced by IL2 is required for dissociation from HRAS and nuclear translocation of IKZF3 in T-cells. Phosphorylation on tyrosine residues induced by IL4 is required for dissociation from Bcl-X(L) in T-cells.
  • 細胞内局在
    Nucleus. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AIO antibody
    • Aiolos antibody
    • IKAROS family zinc finger 3 (Aiolos) antibody
    • IKAROS family zinc finger 3 antibody
    • Ikaros family zinc finger protein 3 antibody
    • IKZF 3 antibody
    • IKZF3 antibody
    • IKZF3_HUMAN antibody
    • zinc finger DNA binding protein Aiolos antibody
    • Zinc finger protein Aiolos antibody
    • Zinc finger protein subfamily 1A 3 (Aiolos) antibody
    • Zinc finger protein subfamily 1A 3 antibody
    • Zinc finger protein subfamily 1A, member 3 antibody
    • ZNFN1A3 antibody
    see all

画像

  • IKZF3 was immunoprecipitated using 0.5mg Raji whole cell extract, 5µg of Rabbit polyclonal to IKZF3 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Raji whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab64400.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 64kDa; IKZF3

  • All lanes : Anti-IKZF3 antibody (ab64400) at 1 µg/ml

    Lane 1 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 64 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa (possible isoform).

    Exposure time : 10 minutesZinc finger protein Aiolos (IKZF3) contains a number of potential phosphorylation sites (SwissProt data) which may explain it running at a higher molecular weight than predicted. IKZF3 has 6 isoforms of differing molecular weight and the 64 kDa band is thought to represent isoform 1 (58 kDa) in a phosphorylated form. The band of a slightly lower intensity seen at 48 kDa, is thought to represent isoform 2 (51 kDA). Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

参考文献

ab64400 has not yet been referenced specifically in any publications.

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