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RabMAb

Anti-IKKi/IKKe 抗体 [EPR4904] (ab124766)

製品の概要

  • 製品名
    Anti-IKKi/IKKe antibody [EPR4904]
    IKKi/IKKe 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4904] to IKKi/IKKe
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
    適用なし: IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide within Human IKKi/IKKe aa 1-100. The exact sequence is proprietary.

  • ポジティブ・コントロール
    • HeLa, Raji, MCF7 and Jurkat cell lysates; Human tonsil tissue.
  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/250.
  • 追加情報
    Is unsuitable for IP.
  • ターゲット情報

    • 機能
      Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death.
    • 組織特異性
      Highly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon.
    • 配列類似性
      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
      Contains 1 protein kinase domain.
    • 翻訳後修飾
      Autophosphorylated.
      Sumoylation by TOPORS upon DNA damage is required for protection of cells against DNA damage-induced cell death. Desumoylated by SENP1.
    • 細胞内局在
      Cytoplasm. Nucleus. Nucleus > PML body. Targeting to PML nuclear bodies upon DNA damage is TOPORS-dependent.
    • Information by UniProt
    • 参照データベース
    • 別名
      • I kappa B kinase epsilon antibody
      • I-kappa-B kinase epsilon antibody
      • IkBKE antibody
      • IKK related kinase epsilon antibody
      • IKK-E antibody
      • IKK-epsilon antibody
      • IKK-i antibody
      • IKKE antibody
      • IKKE_HUMAN antibody
      • IKKepsilon antibody
      • IKKI antibody
      • Inducible I kappa B kinase antibody
      • Inducible I kappa-B kinase antibody
      • Inducible IkappaB kinase antibody
      • Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon antibody
      • Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, epsilon antibody
      • Inhibitor of nuclear factor kappa-B kinase subunit epsilon antibody
      • KIAA0151 antibody
      • MGC125294 antibody
      • MGC125295 antibody
      • MGC125297 antibody
      see all

    画像

    • All lanes : Anti-IKKi/IKKe antibody [EPR4904] (ab124766) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 80 kDa

    • ab124766, at 1/100 dilution, staining IKKi/IKKe in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    参考文献

    ab124766 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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