製品の概要

  • 製品名Anti-IKKi/IKKe antibody [EPR4904]
    IKKi/IKKe 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4904] to IKKi/IKKe
  • アプリケーション適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
    適用なし: IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human IKKi/IKKe.

  • ポジティブ・コントロール
    • HeLa, Raji, MCF7 and Jurkat cell lysates; Human tonsil tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death.
    • 組織特異性Highly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon.
    • 配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
      Contains 1 protein kinase domain.
    • 翻訳後修飾Autophosphorylated.
      Sumoylation by TOPORS upon DNA damage is required for protection of cells against DNA damage-induced cell death. Desumoylated by SENP1.
    • 細胞内局在Cytoplasm. Nucleus. Nucleus > PML body. Targeting to PML nuclear bodies upon DNA damage is TOPORS-dependent.
    • Information by UniProt
    • 参照データベース
    • 別名
      • I kappa B kinase epsilon antibody
      • I-kappa-B kinase epsilon antibody
      • IkBKE antibody
      • IKK related kinase epsilon antibody
      • IKK-E antibody
      • IKK-epsilon antibody
      • IKK-i antibody
      • IKKE antibody
      • IKKE_HUMAN antibody
      • IKKepsilon antibody
      • IKKI antibody
      • Inducible I kappa B kinase antibody
      • Inducible I kappa-B kinase antibody
      • Inducible IkappaB kinase antibody
      • Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon antibody
      • Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, epsilon antibody
      • Inhibitor of nuclear factor kappa-B kinase subunit epsilon antibody
      • KIAA0151 antibody
      • MGC125294 antibody
      • MGC125295 antibody
      • MGC125297 antibody
      see all

    Anti-IKKi/IKKe antibody [EPR4904] 画像

    • All lanes : Anti-IKKi/IKKe antibody [EPR4904] (ab124766) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 80 kDa
    • ab124766, at 1/100 dilution, staining IKKi/IKKe in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-IKKi/IKKe antibody [EPR4904] (ab124766) 使用論文

    ab124766 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab124766.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"