製品の概要

  • 製品名
    Anti-IKKi/IKKe antibody [72B587]
    IKKi/IKKe 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [72B587] to IKKi/IKKe
  • アプリケーション
    適用あり: Flow Cyt, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to amino acid residues 175-188, 525-540, or 567-580 of human IKKi/IKKe.

  • ポジティブ・コントロール
    • Daudi whole-cell extract can be used as a positive control for this antibody. IF/ICC: Jeg3 cell line

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab12142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa). For optimal results, primary antibody incubations should be performed at room temperature.
ICC/IF 1/200.

ターゲット情報

  • 機能
    Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death.
  • 組織特異性
    Highly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon.
  • 配列類似性
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Autophosphorylated.
    Sumoylation by TOPORS upon DNA damage is required for protection of cells against DNA damage-induced cell death. Desumoylated by SENP1.
  • 細胞内局在
    Cytoplasm. Nucleus. Nucleus > PML body. Targeting to PML nuclear bodies upon DNA damage is TOPORS-dependent.
  • Information by UniProt
  • 参照データベース
  • 別名
    • I kappa B kinase epsilon antibody
    • I-kappa-B kinase epsilon antibody
    • IkBKE antibody
    • IKK related kinase epsilon antibody
    • IKK-E antibody
    • IKK-epsilon antibody
    • IKK-i antibody
    • IKKE antibody
    • IKKE_HUMAN antibody
    • IKKepsilon antibody
    • IKKI antibody
    • Inducible I kappa B kinase antibody
    • Inducible I kappa-B kinase antibody
    • Inducible IkappaB kinase antibody
    • Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon antibody
    • Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, epsilon antibody
    • Inhibitor of nuclear factor kappa-B kinase subunit epsilon antibody
    • KIAA0151 antibody
    • MGC125294 antibody
    • MGC125295 antibody
    • MGC125297 antibody
    see all

画像

  • ICC/IF image of ab12142 stained Jeg3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12142, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM



  • Predicted band size : 80 kDa

    IKK iota/IKK epsilon (80 kDa) detection by Western blot. The analysis of 10 µg of total cell extract from Daudi cells with the anti-IKK iota/IKK epsilon at 0.5 µg/ml (lane 1) and 3 µg/ml (lane 2) dilution.

    IKKi/IKKen (80 kDa) detection by Western blot. The analysis of 10 µg of total cell extract from Daudi cells with the anti-IKK iota/IKK epsilon at 0.5 µg/ml (lane 1) and 3 µg/ml (lane 2) dilution.
  • Overlay histogram showing Jurkat cells stained with ab12142 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12142, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

参考文献

ab12142 has not yet been referenced specifically in any publications.

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