Anti-IKK gamma (phospho S31) 抗体 (ab55342)

製品の概要

  • 製品名Anti-IKK gamma (phospho S31) antibody
    IKK gamma 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IKK gamma (phospho S31)
  • 特異性This antibody detects endogenous levels of IKK gamma only when phosphorylated at serine 31.
  • アプリケーション適用あり: IHC-P, ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human IKK gamma around the phosphorylation site of serine 31 (E-E-SP-P-L).

  • ポジティブ・コントロール
    • Extracts from 293 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab55342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100.
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

ターゲット情報

  • 機能Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity (By similarity). Essential for viral activation of IRF3.
  • 組織特異性Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 関連疾患Defects in IKBKG are the cause of ectodermal dysplasia anhidrotic with immunodeficiency X-linked (EDAID) [MIM:300291]; also known as hypohidrotic ectodermal dysplasia with immunodeficiency (HED-ID). Is a form of ectoderma dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. Characterized by absence of sweat glands, sparse scalp hair, rare conical teeth and immunological abnormalities resulting in severe infectious diseases.
    Defects in IKBKG are the cause of ectodermal dysplasia anhidrotic with immunodeficiency-osteopetrosis-lymphedema (OLEDAID) [MIM:300301].
    Defects in IKBKG are a cause of immunodeficiency NEMO-related without anhidrotic ectodermal dysplasia (NEMOID) [MIM:300584]; also called immunodeficiency without anhidrotic ectodermal dysplasia, isolated immunodeficiency or pure immunodeficiency. Patients manifest immunodeficiency not associated with other abnormalities, and resulting in increased infection susceptibility. Patients suffer from multiple episodes of infectious diseases.
    Defects in IKBKG are the cause of susceptibility to X-linked familial atypical micobacteriosis type 1 (AMCBX1) [MIM:300636]; also known as X-linked disseminated atypical mycobacterial infection type 1 or X-linked susceptibility to mycobacterial disease type 1. AMCBX1 is the X-linked recessive form of mendelian susceptibility to mycobacterial disease (MSMD). MSMD is a congenital syndrome resulting in predisposition to clinical disease caused by weakly virulent mycobacterial species, such as bacillus Calmette-Guerin vaccines and non-tuberculous, environmental mycobacteria. Patients are also susceptible to the more virulent species Mycobacterium tuberculosis.
    Defects in IKBKG are the cause of recurrent isolated invasive pneumococcal disease type 2 (IPD2) [MIM:300640]. Recurrent invasive pneumococcal disease (IPD) is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD.
    Defects in IKBKG are the cause of incontinentia pigmenti (IP) [MIM:308300]; formerly designed familial incontinentia pigmenti type II (IP2). IP is a genodermatosis usually prenatally lethal in males. In affected females, it causes abnormalities of the skin, hair, eyes, nails, teeth, skeleton, heart, and central nervous system. The prominent skin signs occur in four classic cutaneous stages: perinatal inflammatory vesicles, verrucous patches, a distinctive pattern of hyperpigmentation and dermal scarring.
  • 配列類似性Contains 1 C2HC-type zinc finger.
  • ドメインThe leucine-zipper domain and the C2HC-type zinc-finger are essential for polyubiquitin binding and for the activation of IRF3.
  • 翻訳後修飾Phosphorylation at Ser-68 attenuates aminoterminal homodimerization.
    Polyubiquitinated on Lys-285 through 'Lys-63'; the ubiquitination is mediated by NOD2 and RIPK2 and probably plays a role in signaling by facilitating interactions with ubiquitin domain-containing proteins and activates the NF-kappa-B pathway. Polyubiquitinated on Lys-399 through 'Lys-63'; the ubiquitination is mediated by BCL10, MALT1 and TRAF6 and probably plays a role in signaling by facilitating interactions with ubiquitin domain-containing proteins and activates the NF-kappa-B pathway. Monoubiquitinated on Lys-277 and Lys-309; promotes nuclear export. Linear polyubiquitinated on Lys-285; the head-to-tail polyubiquitination is mediated by the LUBAC complex. Linear polyubiquitinated on Lys-309; the head-to-tail polyubiquitination is mediated by the LUBAC complex.
    Sumoylated on Lys-277 and Lys-309 by SUMO1; the modification results in phosphorylation of Ser-85 by ATM leading to a replacement of the sumoylation by mono-ubiquitination on these residues.
  • 細胞内局在Cytoplasm. Nucleus. Sumoylated NEMO accumulates in the nucleus in response to genotoxic stress.
  • Information by UniProt
  • 参照データベース
  • 別名
    • IkB kinase associated protein 1 antibody
    • IkB kinase subunit gamma antibody
    • Inhibitor of nuclear factor kappa B kinase subunit gamma antibody
    • AMCBX1 antibody
    • FIP 3 antibody
    • FIP-3 antibody
    • FIP3 antibody
    • Fip3p antibody
    • I kappa B kinase gamma antibody
    • I-kappa-B kinase subunit gamma antibody
    • IkB kinase gamma subunit antibody
    • IkB kinase subunit gamma antibody
    • IkB kinase-associated protein 1 antibody
    • Ikbkg antibody
    • IKK-gamma antibody
    • IKKAP1 antibody
    • IKKG antibody
    • IMD33 antibody
    • Incontinentia pigmenti antibody
    • Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase gamma antibody
    • Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, gamma antibody
    • Inhibitor of nuclear factor kappa-B kinase subunit gamma antibody
    • IP antibody
    • IP1 antibody
    • IP2 antibody
    • IPD2 antibody
    • NEMO antibody
    • NEMO_HUMAN antibody
    • NF kappa B essential modifier antibody
    • NF kappa B essential modulator antibody
    • NF-kappa-B essential modifier antibody
    • NF-kappa-B essential modulator antibody
    • ZC2HC9 antibody
    see all

Anti-IKK gamma (phospho S31) antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue section labeling IKK gamma (phospho S31) with ab55342. The image on the right is blocked with the phospho peptide.

  • All lanes : Anti-IKK gamma (phospho S31) antibody (ab55342) at 1/500 dilution

    Lane 1 : Extracts from 293 cells treated with TNF alpha (20ng/ml for 5 mins).
    Lane 2 : Extracts from 293 cells treated with TNF alpha (20ng/ml for 5 mins), and with the immunising phosphopeptide.


    Predicted band size : 47 kDa
    Observed band size : 47 kDa

Anti-IKK gamma (phospho S31) antibody (ab55342) 使用論文

ab55342 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (macrophage)
Specification macrophage
Treatment 0.1 ug/ml LPS 15 min
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Dec 12 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"