製品の概要

  • 製品名Anti-IKK beta antibody
    IKK beta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IKK beta
  • 特異性Control peptide (ab 7612) will compete only with the specific reaction of antiserum with the IKKb subunit.
  • アプリケーション適用あり: WB, IP, ELISA, Dot blotmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to IKK beta (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • ポジティブ・コントロール
    • HeLa cell lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab7611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
ELISA
Dot blot
  • 追加情報Dot: Use at an assay dependant dilution.
    ELISA: Use at an assay dependant dilution.
    IP: Use at an assay dependant dilution.
    WB: 1/1000. Detects a band of approximately 87 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.
    • 組織特異性Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
    • 配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
      Contains 1 protein kinase domain.
    • 翻訳後修飾Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response.
      Acetylation of Thr-180 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway.
      Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. According to PubMed:19675099, 'Ser-163' does not serve as a monoubiquitination site. According to PubMed:16267042, ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues. Monoubiquitination by TRIM21 is dirupted by Yersinia yopJ.
    • 細胞内局在Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
    • Information by UniProt
    • 参照データベース
    • 別名
      • I kappa B kinase 2 antibody
      • I kappa B kinase beta antibody
      • I-kappa-B kinase 2 antibody
      • I-kappa-B-kinase beta antibody
      • IkBKB antibody
      • IKK beta antibody
      • IKK-B antibody
      • IKK-beta antibody
      • IKK2 antibody
      • IKKB antibody
      • IKKB_HUMAN antibody
      • IMD15 antibody
      • Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta antibody
      • Inhibitor of nuclear factor kappa-B kinase subunit beta antibody
      • NFKBIKB antibody
      • Nuclear factor NF-kappa-B inhibitor kinase beta antibody
      see all

    Anti-IKK beta antibody (ab7611) 使用論文

    This product has been referenced in:
    • Tang Y  et al. Inhibition of the IKK/NF-?B pathway by AAV gene transfer improves muscle regeneration in older mdx mice. Gene Ther 17:1476-83 (2010). Read more (PubMed: 20720575) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry. The 80 mg/ml is the total protein concentration (as determined by Refractometry) and is not the antibody concentration. The purity of this product is indeed whole antiserum and as it is unpurified, an exact antibody concentr...

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    I'm sorry to hear that you are having problems with ab7611 in WB. I would recommend removing the milk from your protocol as this may be the source of the non-specific binding and to increase the amount of antibody (trying 1:300-1:500) as the diluti...

    Read More

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"