遺伝子ノックアウト細胞株 検証済
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RabMAb

Anti-IKB beta 抗体 [EPR5037] (ab109509)

製品の概要

  • 製品名
    Anti-IKB beta antibody [EPR5037]
    IKB beta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5037] to IKB beta
  • アプリケーション
    適用あり: WB, IP, IHC-P, ICCmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues on the C-terminus in Human IKB beta.

  • ポジティブ・コントロール
    • Jurkat, HeLa, THP-1, and MCF7 cell lysates, Human kidney tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 37 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • 追加情報
    Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能
      Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.
    • 組織特異性
      Expressed in all tissues examined.
    • 配列類似性
      Belongs to the NF-kappa-B inhibitor family.
      Contains 6 ANK repeats.
    • 翻訳後修飾
      Phosphorylated by RPS6KA1; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation.
    • 細胞内局在
      Cytoplasm. Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • I kappa B beta antibody
      • I-kappa-B-beta antibody
      • IkappaBbeta antibody
      • IKB beta antibody
      • IkB-B antibody
      • IkB-beta antibody
      • IKBB antibody
      • IKBB_HUMAN antibody
      • IkBbeta antibody
      • NF kappa BIB antibody
      • NF-kappa-B inhibitor beta antibody
      • NF-kappa-BIB antibody
      • Nfkbib antibody
      • Thyroid receptor interacting protein 9 antibody
      • Thyroid receptor-interacting protein 9 antibody
      • TR interacting protein 9 antibody
      • TR-interacting protein 9 antibody
      • TRIP-9 antibody
      • TRIP9 antibody
      see all

    Anti-IKB beta antibody [EPR5037] 画像



    • Predicted band size : 37 kDa

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: IKB beta knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)

      Lanes 1 - 3: Merged signal (red and green). Green - ab109509 observed at 45 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab109509 was shown to specifically react with IKB beta in wild type cells as signal was lost in IKB beta knockout cells. Wild-type and IKB beta knockout samples were subjected to SDS-PAGE. ab109509 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ab109509 at a dilution of 1/100.
    • All lanes : Anti-IKB beta antibody [EPR5037] (ab109509) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : THP-1 cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 37 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)

    Anti-IKB beta antibody [EPR5037] (ab109509) 使用論文

    ab109509 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab109509.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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