製品の概要

  • 製品名Anti-IKB beta antibody
    IKB beta 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to IKB beta
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Rat
    交差が予測される動物種: Mouse
  • 免疫原

    Recombinant fusion protein (tagged): EKLYAAGAGV LVTERGGHTA LHLACRVRAH TCAYVLLQPR PSHPRDASDT YLTQSQDHTP DTSHAPVATD PQPNPGNEEE LRDEDWRLQL EAENYDGHTP , corresponding to amino acids 111-210 of Rat IKK beta

  • 特記事項


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000.

ターゲット情報

  • 機能Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.
  • 組織特異性Expressed in all tissues examined.
  • 配列類似性Belongs to the NF-kappa-B inhibitor family.
    Contains 6 ANK repeats.
  • 翻訳後修飾Phosphorylated by RPS6KA1; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • I kappa B beta antibody
    • I-kappa-B-beta antibody
    • IkappaBbeta antibody
    • IKB beta antibody
    • IkB-B antibody
    • IkB-beta antibody
    • IKBB antibody
    • IKBB_HUMAN antibody
    • IkBbeta antibody
    • NF kappa BIB antibody
    • NF-kappa-B inhibitor beta antibody
    • NF-kappa-BIB antibody
    • Nfkbib antibody
    • Thyroid receptor interacting protein 9 antibody
    • Thyroid receptor-interacting protein 9 antibody
    • TR interacting protein 9 antibody
    • TR-interacting protein 9 antibody
    • TRIP-9 antibody
    • TRIP9 antibody
    see all

Anti-IKB beta antibody 画像

  • All lanes : Anti-IKB beta antibody (ab52775) at 1/1000 dilution

    Lane 1 : a total protein extract from E coli with 50ng to 100 ng of a tagged fusion protein of an irrelevant antigen.
    Lane 2 : a total protein extract from E coli with 50ng to 500ng of the antigen (tagged antigen fusion protein)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase at 1/5000 dilution

    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

Anti-IKB beta antibody (ab52775) 使用論文

ab52775 has not yet been referenced specifically in any publications.

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