製品の概要

  • 製品名Anti-IKB alpha antibody
    IKB alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IKB alpha
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human, Pig
    交差が予測される動物種: Monkey, Gorilla, Gerbil
  • 免疫原

    Synthetic peptide:

    TESEFTEFTEDELPYDDCVFGGQRLTL

    conjugated to KLH, corresponding to C terminal amino acids 297-317 (with N-terminally added lysine)of Human IKB alpha.

  • ポジティブ・コントロール
    • whole extract from a human epitheloid carcinoma HeLa cell line. whole extract from the human epidermal carcinoma A431 cell line.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab49978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
AP Use at an assay dependent dilution.
WB 1/2000. Predicted molecular weight: 36 kDa.

ターゲット情報

  • 機能Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
  • 関連疾患Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant
  • 配列類似性Belongs to the NF-kappa-B inhibitor family.
    Contains 5 ANK repeats.
  • 翻訳後修飾Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
    Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
    Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
    Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.
  • 細胞内局在Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
  • Information by UniProt
  • 参照データベース
  • 別名
    • I kappa B alpha antibody
    • I-kappa-B-alpha antibody
    • IkappaBalpha antibody
    • IkB-alpha antibody
    • IKBA antibody
    • IKBA_HUMAN antibody
    • IKBalpha antibody
    • MAD 3 antibody
    • MAD3 antibody
    • Major histocompatibility complex enhancer-binding protein MAD3 antibody
    • NF kappa B inhibitor alpha antibody
    • NF-kappa-B inhibitor alpha antibody
    • NFKBI antibody
    • NFKBIA antibody
    • Nuclear factor of kappa light chain gene enhancer in B cells antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody
    see all

Anti-IKB alpha antibody (ab49978) 使用論文

This product has been referenced in:
  • Caperna TJ  et al. Identification and characterization of a nuclear factor-? B-p65 proteolytic fragment in nuclei of porcine hepatocytes in monolayer culture. Domest Anim Endocrinol 45:154-62 (2013). WB ; Pig . Read more (PubMed: 24011531) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Cow Cell lysate - whole cell (macrophage)
Loading amount 10 µg
Specification macrophage
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Apr 07 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"