Anti-IKAP 抗体 - Carboxyterminal end (ab62498)

製品の概要

  • 製品名
    Anti-IKAP antibody - Carboxyterminal end
    IKAP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IKAP - Carboxyterminal end
  • 特異性
    This antibody reacts with IKAP
  • アプリケーション
    適用あり: WB, ICC, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A 16 amino acid synthetic peptide from near the carboxy terminus of human IKAP.

  • ポジティブ・コントロール
    • A20 cell lysate and A20 cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 148, 100 kDa (predicted molecular weight: 148 kDa).
ICC Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.

ターゲット情報

  • 機能
    May act as a scaffold protein that may assemble active IKK-MAP3K14 complexes (IKKA, IKKB and MAP3K14/NIK).
    Acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4.
  • 関連疾患
    Defects in IKBKAP are the cause of hereditary sensory and autonomic neuropathy type 3 (HSAN3) [MIM:223900]; also known as Riley-Day syndrome or familial dysautonomia (FD). This autosomal recessive disorder is due to the poor development and survival, and progressive degeneration of the sensory, sympathetic and parasympathetic neurons. HSAN3 individuals are affected with a variety of symptoms such as decreased sensitivity to pain and temperature, cardiovascular instability, recurrent pneumonias, vomiting crises, and gastrointestinal dysfunction. It is primarily confined to individuals of Ashkenazi Jewish descent, with an incidence of 1/3'600 live births.
  • 配列類似性
    Belongs to the ELP1/IKA1 family.
  • 細胞内局在
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp781H1425 antibody
    • DYS antibody
    • Dysautonomia (Riley Day syndrome hereditary sensory autonomic neuropathy type III) antibody
    • Elongator complex protein 1 antibody
    • ELP 1 antibody
    • ELP1 antibody
    • ELP1_HUMAN antibody
    • FD antibody
    • FLJ12497 antibody
    • IKAP antibody
    • IkappaB kinase complex associated protein antibody
    • IkappaB kinase complex-associated protein antibody
    • ikbkap antibody
    • IKI 3 antibody
    • IKI3 antibody
    • IKK complex associated protein antibody
    • IKK complex-associated protein antibody
    • Inhibitor of kappa light polypeptide gene enhancer in B cells kinase complex associated protein antibody
    • OTTHUMP00000063889 antibody
    • p150 antibody
    • TOT 1 antibody
    • TOT1 antibody
    see all

画像

  • Lane 1 : Anti-IKAP antibody - Carboxyterminal end (ab62498) at 0.5 µg/ml
    Lane 2 : Anti-IKAP antibody - Carboxyterminal end (ab62498) at 1 µg/ml

    Lane 1 : A20 cell lysate
    Lane 2 : A20 cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    anti-rabbit IgG

    Predicted band size : 148 kDa
    Observed band size : 100,148 kDa (why is the actual band size different from the predicted?)
  • Immunocytochemistry of IKAP in A20 cells with ab62498 antibody at 1 µg/ml.
  • Immunofluorescence of IKAP in A20 cells using ab62498 at 20 ug/ml.

参考文献

ab62498 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (testis)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
testis
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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投稿 Dec 04 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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