製品の概要

  • 製品名
    Anti-IGSF1 antibody
  • 製品の詳細
    Rabbit polyclonal to IGSF1
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide: CNVNILCHGW LQDLVFMLFK EGYAEPVDYQ VPTGTMAIFS IDNLTPEDEG , corresponding to amino acids 146-195 of Human IGSF1

  • ポジティブ・コントロール
    • WB: HepG2 cell lysate IHC-P: Human kidney tissue

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab66509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 148 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 関連性
    IGSF1 is a member of the immunoglobulin (Ig) superfamily. They have a variety of functions, but all appear to play a role in cell recognition and the regulation of cell behavior.
  • 細胞内局在
    Isoforms 1 and 2: Membrane; Multipass membrane protein (Potential). Isoform 3: Secreted (Potential).
  • 参照データベース
  • 別名
    • IGCD1 antibody
    • IGDC1 antibody
    • Immunoglobulin like domain containing protein 1 antibody
    • Immunoglobulin superfamily member 1 antibody
    • InhBP antibody
    • Inhibin binding protein antibody
    • KIAA0364 antibody
    • MGC75490 antibody
    • p120 antibody
    • PGSF2 antibody
    • Pituitary gland specific factor 2 antibody
    see all

画像

  • Anti-IGSF1 antibody (ab66509) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 148 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling IGSF1 with ab66509 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

参考文献

This product has been referenced in:

See 1 Publication for this product

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