Anti-IgM 抗体 [UCH-B1] (ab18201)
Key features and details
- Mouse monoclonal [UCH-B1] to IgM
- Suitable for: ICC/IF, IHC-Fr, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-IgM antibody [UCH-B1]
IgM 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [UCH-B1] to IgM -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, IHC-Fr, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human IgM. Human prolymphocytic leukaemia B cells.
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特記事項
UCH-B1 antigen (85kD) is detected on a sub-population of B lymphocytes, probably related to stage of activation or maturation. UCH-B1 may be useful for differential diagnosis of B cell leukaemias and as a marker of prolymphocytic leukaemia.
Does not work on formalin fixed and parrafin embedded tissues.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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精製度
Protein A/G purified -
一次抗体 備考
UCH-B1 antigen (85kD) is detected on a sub-population of B lymphocytes, probably related to stage of activation or maturation. UCH-B1 may be useful for differential diagnosis of B cell leukaemias and as a marker of prolymphocytic leukaemia. -
ポリ/モノ
モノクローナル -
クローン名
UCH-B1 -
ミエローマ
NS0 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab18201の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
Acetone fixed. |
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. Acetone fixed. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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関連性
IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences. Class specific anti immunoglobulin antibodies are useful for: The characterization of malignant B cell proliferations. All but acute lymphocytic leukemias share either surface or intra cytoplasmic Ig with an isotypic restriction, which suggest the monoclonal nature of the cell population. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt's lymphoma bear surface IgM, whereas plasmocytes from Waldenström's disease bear intracytoplasmic IgM. The other isotypes are less frequently found. On the other hand multiple myelomas are usually of the IgG or IgA type. Characterization of plasma cells in inflammatory conditions: Plasma cell typing can be of use for the classification of intestinal inflammatory conditions such as inflammatory bowel disease and allergic conditions. In the latter a specific increase in the number of IgE plasma cells can be demonstrated. -
細胞内局在
Isoform 1: Secreted. During differentiation, B lymphocytes switch from expression of membrane bound IgM to secretion of IgM. Isoform 2: Cell membrane; Single pass type I membrane protein. -
参照データベース
- Entrez Gene: 3507 Human
- Omim: 147020 Human
- SwissProt: P01871 Human
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別名
- AGM1 antibody
- Constant region of heavy chain of IgM antibody
- DKFZp686I15196 antibody
see all
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab18201 は 4 報の論文で使用されています。
- Grupp SA et al. Molecular mechanisms that control expression of the B lymphocyte antigen receptor complex. J Exp Med 181:161-8 (1995). PubMed: 7807000
- DeFranco AL et al. Structure and function of the B-cell antigen receptor. Chem Immunol 59:156-72 (1994). PubMed: 7945925
- Smith-Ravin J et al. Characterization of two monoclonal antibodies (UCL4D12 and UCL3D3) that discriminate between human mantle zone and marginal zone B cells. Clin Exp Immunol 82:181-7 (1990). PubMed: 2208792
- Armitage RJ et al. A new antigen identified by the monoclonal antibody UCHB 1 delivers a costimulatory signal to a subset of human B cells. Eur J Immunol 18:67-76 (1988). PubMed: 3257923