製品の概要

  • 製品名Anti-IgG4 antibody [EP4420]
    IgG4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP4420] to IgG4
  • 特異性Does not cross-react with IgG1, IgG2, or IgG3.
  • アプリケーション適用あり: WB, IHC-Pmore details
    適用なし: ICC or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues in the Hinge region of Human IgG4.

  • ポジティブ・コントロール
    • Human spleen and Human plasma lysates; Human tonsil tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EP4420
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab109493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • 追加情報Is unsuitable for ICC or IP.
  • ターゲット情報

    • 関連性IgG4 antibodies will dominate the IgG response in schistosomiasis, lymphatic filariasis, and in patients after allergen immunotherapy. Unlike the other IgG subclasses, IgG4 does not activate complement. A combined IgA-IgG4 deficiency has been associated with recurrent pyogenic infections.
    • 細胞内局在Secreted
    • 参照データベース
    • 別名
      • Ig gamma 4 chain C region antibody
      • IGHG4 antibody
      • Immunoglobulin heavy constant gamma 4 (G4m marker) antibody
      • MGC117419 antibody

    Anti-IgG4 antibody [EP4420] 画像

    • ab109493 at 1/1000 dilution staining IgG4 in Paraffin embedded Human tonsil tissue.
    • All lanes : Anti-IgG4 antibody [EP4420] (ab109493) at 1/1000 dilution

      Lane 1 : IgG4 Recombinant protein at 0.005 µg
      Lane 2 : IgG1 Recombinant protein at 0.01 µg
      Lane 3 : IgG2 Recombinant protein at 0.01 µg
      Lane 4 : IgG3 Recombinant protein at 0.01 µg
      Lane 5 : Human spleen lysate at 10 µg
      Lane 6 : Human plasma lysate at 10 µg


      Predicted band size : 36 kDa

    Anti-IgG4 antibody [EP4420] (ab109493) 使用論文

    This product has been referenced in:
    • Ma JM  et al. Clinical research on benign lymphoepithelial lesions of lacrimal gland in 20 Chinese patients. Chin Med J (Engl) 128:493-8 (2015). IHC-P ; Human . Read more (PubMed: 25673452) »
    • Zhang R  et al. Clinicopathological analysis of 12 patients with autoimmune pancreatitis. Exp Ther Med 10:121-126 (2015). IHC-P ; Human . Read more (PubMed: 26170922) »

    See all 3 Publications for this product

    Product Wall

    I have reviewed these products and have been in touch with the labs regarding reactivity with Rhesus as requested. We would not recommend any human IgG2 antibody which we know is directed to the hinge region, such as ab1935, against Rhesus as the homol...

    Read More

    Gracias por tu respuesta.
    Este es un mail de confirmación del nuevo pedido, consistente en el reemplazo completamente gratuito, del anticuerpo ab99466 y el envío de ab109493 como parte de la promoción para testar anticuerpos RabMab. El número de p...

    Read More

    Thank you for contacting Abcam.

    This product is expected to remain stable for up to 1 year from receipt when stored properly at -20oC.

    I hope this information is helpful. Please do not hesitate to contact us if you have any additi...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"