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RabMAb

Anti-IgG1 抗体 [EPR4417] (ab108969)

製品の概要

  • 製品名
    Anti-IgG1 antibody [EPR4417]
    IgG1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4417] to IgG1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    corresponding to Human IgG1 (Hinge).

  • ポジティブ・コントロール
    • Human tonsil and human spleen lysates.
  • 特記事項

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • 解離定数(KD 値)
    KD = 3.87 x 10 -10 M Learn more about KD
  • バッファー
    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    EPR4417
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab108969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.

ターゲット情報

  • 関連性
    There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant).
  • 細胞内局在
    Secreted. Cell membrane; Single-pass membrane protein
  • 参照データベース
  • 別名
    • Ig gamma 1 chain C region antibody
    • IgG heavy chain locus antibody
    • IGHG1 antibody
    • Immunoglobulin Gm1 antibody
    • Immunoglobulin heavy constant gamma 1 (G1m marker) antibody
    see all

Anti-IgG1 antibody [EPR4417] 画像

  • All lanes : Anti-IgG1 antibody [EPR4417] (ab108969) at 1/10000 dilution (purified)

    Lane 1 : Human tonsil lysate
    Lane 2 : Human spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size : 36 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)

    Blocking and diluting buffer: 5% NFDM/TBST.

  • All lanes : Anti-IgG1 antibody [EPR4417] (ab108969) at 1/1000 dilution (unpurified)

    Lane 1 : IgG1 at 0.01 µg
    Lane 2 : IgG2 at 0.01 µg
    Lane 3 : IgG3 at 0.01 µg
    Lane 4 : IgG4 at 0.01 µg
    Lane 5 : Human tonsil lysate at 10 µg
    Lane 6 : Human spleen lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 36 kDa
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

プロトコール

Anti-IgG1 antibody [EPR4417] (ab108969) 使用論文

ab108969 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab108969.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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