Anti-IgG 抗体 [R10Z8E9] (ab124055)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [R10Z8E9] to IgG
- Suitable for: Indirect ELISA, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-IgG antibody [R10Z8E9]
IgG 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [R10Z8E9] to IgG -
由来種
Mouse -
アプリケーション
適用あり: Indirect ELISA, WBmore details
適用なし: IHC-P -
種交差性
交差種: Human -
免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Indirect ELISA: Human IgG1. WB: Human serum lysate.
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特記事項
This product has switched from a hybridoma to recombinant production method on 21st September 2020.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59.04% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
R10Z8E9 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124055の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Indirect ELISA |
Use at an assay dependent concentration.
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WB |
1/1000. Predicted molecular weight: 36 kDa.
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特記事項 |
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Indirect ELISA
Use at an assay dependent concentration. |
WB
1/1000. Predicted molecular weight: 36 kDa. |
ターゲット情報
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細胞内局在
Secreted -
参照データベース
- Entrez Gene: 3500 Human
- Entrez Gene: 3501 Human
- Entrez Gene: 3502 Human
- Entrez Gene: 3503 Human
- SwissProt: P01857 Human
- SwissProt: P01859 Human
- SwissProt: P01860 Human
- SwissProt: P01861 Human
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別名
- Ig gamma 1 chain C region antibody
- Ig gamma 2 chain C region antibody
- Ig gamma 3 chain C region antibody
see all
画像
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Anti-IgG antibody [R10Z8E9] (ab124055) at 1/1000 dilution + Human serum lysate at 10 µg
Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366)
Predicted band size: 36 kDa
Exposure time: 3 minutesBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Expected MW: 50 kDa.
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50ng Human IgG1 (50μL at 1000ng/mL) was coated onto 96-wells. Serial dilutions (0, 0.001, 0.005, 0.020, 0.078, 0.313, 1.25, 5μg/mL) of ab124055 (50μL) were incubated with Human IgG1 for 60 minutes by shaking. This was followed by adding goat anti-mouse IgG, (H+L), phosphatase-conjugated secondary antibody 1/1000 diluted (50μL) into each well and incubating for another 40 minutes. After washing, 50 μL of PNPP was added and incubated for 13 minutes without shaking for color development. OD was read at 405nm within 5 minutes.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab124055 は 5 報の論文で使用されています。
- Wang H et al. AADAC promotes therapeutic activity of cisplatin and imatinib against ovarian cancer cells. Histol Histopathol 37:899-907 (2022). PubMed: 35451495
- Chi Y et al. Exosomal lncRNA UCA1 Derived From Pancreatic Stellate Cells Promotes Gemcitabine Resistance in Pancreatic Cancer via the SOCS3/EZH2 Axis. Front Oncol 11:671082 (2021). PubMed: 34868904
- Hou L et al. Concurrent binding to DNA and RNA facilitates the pluripotency reprogramming activity of Sox2. Nucleic Acids Res 48:3869-3887 (2020). PubMed: 32016422
- Zhang J et al. RE1-silencing transcription factor controls the acute-to-chronic neuropathic pain transition and Chrm2 receptor gene expression in primary sensory neurons. J Biol Chem 293:19078-19091 (2018). PubMed: 30327427
- Laumet G et al. G9a is essential for epigenetic silencing of K(+) channel genes in acute-to-chronic pain transition. Nat Neurosci 18:1746-55 (2015). PubMed: 26551542