製品の概要

  • 製品名Anti-IGFBP7 antibody
    IGFBP7 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IGFBP7
  • アプリケーション適用あり: ICC/IF, WB, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Rainbow Trout
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human IGFBP7.

    (Peptide available as ab85821.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: Mouse Spinal Cord Tissue, Human Brain Tissue, Rat Brain Tissue, EBS E14 TG2A Day 3 Whole Cell

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab74169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 - 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 29 kDa).
IHC-Fr Use at an assay dependent concentration. PubMed: 21873981

ターゲット情報

  • 機能Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion.
  • 配列類似性Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 Kazal-like domain.
  • 翻訳後修飾N-glycosylated.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AGM antibody
    • Angiomodulin antibody
    • FSTL2 antibody
    • IBP-7 antibody
    • IBP7 antibody
    • IBP7_HUMAN antibody
    • IGF binding protein 7 antibody
    • IGF-binding protein 7 antibody
    • IGFBP rP1 antibody
    • IGFBP-7 antibody
    • IGFBP-rP1 antibody
    • IGFBP7 antibody
    • IGFBPRP1 antibody
    • Insulin like growth factor binding protein 7 antibody
    • Insulin-like growth factor-binding protein 7 antibody
    • MAC25 antibody
    • MAC25 protein antibody
    • PGI2 stimulating factor antibody
    • PGI2-stimulating factor antibody
    • Prostacyclin stimulating factor antibody
    • Prostacyclin-stimulating factor antibody
    • PSF antibody
    • RAMSVPS antibody
    • TAF antibody
    • Tumor-derived adhesion factor antibody
    see all

Anti-IGFBP7 antibody 画像

  • Anti-IGFBP7 antibody (ab74169) at 1 µg/ml + Recombinant Human IGFBP7 protein (ab50195) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 29 kDa


    Exposure time : 30 seconds

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab74169 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • All lanes : Anti-IGFBP7 antibody (ab74169) at 1 µg/ml

    Lane 1 : Spinal Cord (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Human brain tissue lysate - total protein (ab29466)
    Lane 4 : EBS E14 TG2A Day 3 (Mouse Pluripotent Embryonic Stem Cell) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 47 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab74169 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • ICC/IF image of ab74169 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74169, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 1µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 1µg/ml.

Anti-IGFBP7 antibody (ab74169) 使用論文

This product has been referenced in:
  • Contois LW  et al. Inhibition of tumor-associated avß3 integrin regulates the angiogenic switch by enhancing expression of IGFBP-4 leading to reduced melanoma growth and angiogenesis in vivo. Angiogenesis N/A:N/A (2014). Read more (PubMed: 25249331) »
  • Agbemenyah HY  et al. Insulin growth factor binding protein 7 is a novel target to treat dementia. Neurobiol Dis N/A:N/A (2013). WB ; Human, Mouse . Read more (PubMed: 24075854) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Non-Denaturing (Native)
Sample Human Serum (conditioned medium)
Specification conditioned medium
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jan 08 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (15% gel)
Sample Human Purified protein (periodontal ligament cell-conditioned medium)
Specification periodontal ligament cell-conditioned medium
Blocking step Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Oct 29 2014

Thank you for contacting us.
While the immunogen sequences for our antibodies are considered proprietary, we do provide general information about the immunogen range on our datasheets:
ab38972: Synthetic peptide based on the Human sequence. T...

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Thank you for your response.

This is to confirm thatI have placed a new order for you - for one vial ofab74169 as a free of charge replacement and the new order number is1065537 and the item will be shipped next Tuesday (10th April) after Eas...

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Thank you for your response. The discount code has been issued for you for testing this antibody in IP. I am forwarding my colleague's e-mail below so please read the discount offer. If you have any questions, please do not hesitate to contact us again...

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Thank you for your contact regarding ab74169. I am very sorry to hear that you are having problem with this antibody.

I understand from your e-mail this antibody has been used in Western blot and IHC application and it worked initially but fa...

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Thank you for contacting us. The western blot data on our website was generated under reduced and denatured conditions. The antibody may also be suitable for native WB, but we have not validated this in house. I hope this helps, please let me know if y...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (Precipitated protein cell culture medium)
Loading amount 50 µg
Specification Precipitated protein cell culture medium
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Apr 16 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (muscle)
Loading amount 3 µg
Specification muscle
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10% Tris Glycin)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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投稿 Jan 14 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"