Anti-IGFBP5 抗体 (Biotin) (ab84229)

製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
Sandwich ELISA
  • 追加情報
    ELISA: Use at a concentration of 0.25 - 1 µg/ml (100 µl/well).
    sELISA: Use at a concentration of 0.25 - 1 µg/ml (100 µl/well).
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 30 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
    • 組織特異性
      Osteosarcoma, and at lower levels in liver, kidney and brain.
    • 配列類似性
      Contains 1 IGFBP N-terminal domain.
      Contains 1 thyroglobulin type-1 domain.
    • 細胞内局在
      Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • IBP 5 antibody
      • IBP-5 antibody
      • IBP5 antibody
      • IBP5_HUMAN antibody
      • IGF binding protein 5 antibody
      • IGF BP5 antibody
      • IGF-binding protein 5 antibody
      • IGFBP 5 antibody
      • IGFBP-5 antibody
      • IGFBP5 antibody
      • Insulin like growth factor binding protein 5 antibody
      • Insulin-like growth factor-binding protein 5 antibody
      see all

    画像

    • Anti-IGFBP5 antibody (Biotin) (ab84229) at 0.1 µg/ml + Recombinant Human IGFBP5 protein (ab49835) at 0.01 µg

      Secondary
      Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 30 seconds

    プロトコール

    参考文献

    ab84229 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Thank you for your enquiry and your interest.
    I could offer you a testing discount for a pair of antibodies to test them together as a pair in Sandwich ELISA (IGFBP5).
    I would advise you to visit the following site where the Terms and Conditi...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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