製品の概要

  • 製品名Anti-IGFBP4 antibody
    IGFBP4 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to IGFBP4
  • 特異性Based on ELISA and immunoblotting, this antibody shows less than 1 % cross-reactivity with recombinant human IGFBP1 and no cross reactivity with recombinant human IGFBP2 and recombinant human IGFBP3.
  • アプリケーション適用あり: ELISA, WB, Neutralisingmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein (Human).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
Neutralising
  • 追加情報ELISA: Use at a concentration of 0.5 - 1.0 µg/ml. The detection limit for recombinant human IGFBP4 is approximately 0.06 ng/well.
    Neut: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. Detects bands of approximately 25 and 32 kDa. The detection limit for recombinant human IGFBP4 is approximately 2 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively.

    The Neutralization Dose50 (ND50) for anti human IGFBP4 is approximately 10 to 30 µg/ml in the presence of 0.3 µg/ml of recombinant human IGFBP4 and 14 ng/ml of recombinant human IGF-II using the human breast cancer cell line MCF-7 cells. The ND50 is the concentration of antibody required to yield one half maximal inhibition of the cytokine activity on a responsive cell line, when the cytokine is present at a concentration just high enough to elicit a maximum response. The exact concentration of antibody required to neutralize human IGFBP4 activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity studied.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
    • 配列類似性Contains 1 IGFBP N-terminal domain.
      Contains 1 thyroglobulin type-1 domain.
    • 細胞内局在Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AI875747 antibody
      • BP 4 antibody
      • BP4 antibody
      • Deb2 antibody
      • HT29 IGFBP antibody
      • IBP 4 antibody
      • IBP-4 antibody
      • IBP4 antibody
      • IBP4_HUMAN antibody
      • IGF binding protein 4 antibody
      • IGF-binding protein 4 antibody
      • IGF-BP4 antibody
      • IGFBP 4 antibody
      • IGFBP-4 antibody
      • IGFBP4 antibody
      • Insulin Like Growth Factor Binding Protein 4 antibody
      • Insulin-like growth factor-binding protein 4 antibody
      see all

    Anti-IGFBP4 antibody (ab10734) 使用論文

    ab10734 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"