製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to IGFBP3
  • 特異性
    ab77635 is expected to recognize both reported isoforms (NP_001013416.1 and NP_000589.2).
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Sheep, Human, Pig
    交差が予測される動物種: Mouse, Rat, Horse, Cow, Dog
  • 免疫原

    Synthetic peptide:

    C-RYKVDYESQSTDTQN

    , from the internal region of human IGFBP3 (NP_001013416.1; NP_000589.2).

  • ポジティブ・コントロール
    • Human breast cancer lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/64000.
    WB: Use at a concentration of 0.02 - 0.06 µg/ml. Predicted molecular weight: 32 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
    • 組織特異性
      Expressed by most tissues. Present in plasma.
    • 配列類似性
      Contains 1 IGFBP N-terminal domain.
      Contains 1 thyroglobulin type-1 domain.
    • 発生段階
      IGFBP3 levels are higher during extrauterine life and peak during puberty.
    • ドメイン
      The thyroglobulin type-1 domain mediates interaction with HN.
    • 翻訳後修飾
      Phosphorylation sites are present in the extracelllular medium.
    • 細胞内局在
      Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Acid stable subunit of the 140 K IGF complex antibody
      • Binding protein 29 antibody
      • Binding protein 53 antibody
      • BP 53 antibody
      • BP53 antibody
      • Growth hormone dependent binding protein antibody
      • IBP 3 antibody
      • IBP-3 antibody
      • IBP3 antibody
      • IBP3_HUMAN antibody
      • IGF binding protein 3 antibody
      • IGF-binding protein 3 antibody
      • IGFBP 3 antibody
      • IGFBP-3 antibody
      • IGFBP3 antibody
      • Insulin Like Growth Factor Binding Protein 3 antibody
      • Insulin-like growth factor binding protein 3 precursor antibody
      • Insulin-like growth factor-binding protein 3 antibody
      see all

    画像

    • All lanes : Anti-IGFBP3 antibody (ab77635) at 0.02 µg/ml

      Lane 1 : human breast cancer lysate (in RIPA buffer)
      Lane 2 : human breast cancer lysate (in RIPA buffer) with immunizing peptide

      Lysates/proteins at 35 µg per lane.


      Predicted band size : 32 kDa
      Observed band size : 28 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 50 kDa (possible non-specific binding).

    プロトコール

    参考文献

    This product has been referenced in:
    • Marthandan S  et al. Conserved genes and pathways in primary human fibroblast strains undergoing replicative and radiation induced senescence. Biol Res 49:34 (2016). Read more (PubMed: 27464526) »
    • Goyal R  et al. Antenatal maternal long-term hypoxia: acclimatization responses with altered gene expression in ovine fetal carotid arteries. PLoS One 8:e82200 (2013). WB ; Sheep . Read more (PubMed: 24367503) »

    See all 3 Publications for this product

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Sheep Tissue lysate - whole (Liver)
    Loading amount
    3 µg
    Specification
    Liver
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    投稿 Sep 21 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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