Anti-IGFBP3 抗体 (ab77635)
Key features and details
- Goat polyclonal to IGFBP3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-IGFBP3 antibody
IGFBP3 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to IGFBP3 -
由来種
Goat -
特異性
ab77635 is expected to recognize both reported isoforms (NP_001013416.1 and NP_000589.2). -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Horse, Cow, Dog -
免疫原
Synthetic peptide:
C-RYKVDYESQSTDTQN
, from the internal region of human IGFBP3 (NP_001013416.1; NP_000589.2). -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab77635 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77635の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
特記事項 |
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WB: Use at a concentration of 0.02 - 0.06 µg/ml. Predicted molecular weight: 32 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
組織特異性
Expressed by most tissues. Present in plasma. -
配列類似性
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
発生段階
IGFBP3 levels are higher during extrauterine life and peak during puberty. -
ドメイン
The thyroglobulin type-1 domain mediates interaction with HN. -
翻訳後修飾
Phosphorylation sites are present in the extracelllular medium. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3486 Human
- Entrez Gene: 16009 Mouse
- Entrez Gene: 24484 Rat
- Omim: 146732 Human
- SwissProt: P20959 Cow
- SwissProt: P17936 Human
- SwissProt: P47878 Mouse
- SwissProt: P15473 Rat
see all -
別名
- Acid stable subunit of the 140 K IGF complex antibody
- Binding protein 29 antibody
- Binding protein 53 antibody
see all
画像
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All lanes : Anti-IGFBP3 antibody (ab77635) at 0.02 µg/ml
Lane 1 : human breast cancer lysate (in RIPA buffer)
Lane 2 : human breast cancer lysate (in RIPA buffer) with immunizing peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 32 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa (possible non-specific binding)
The additional band of 50 kDa was consistently observed. However this band was not blocked by the immunizing peptide and it is therefore a non specific signal.
データシートおよび資料
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Datasheet download
参考文献 (6)
ab77635 は 6 報の論文で使用されています。
- Nery FC et al. Impaired kidney structure and function in spinal muscular atrophy. Neurol Genet 5:e353 (2019). PubMed: 31517062
- Bai R et al. The NF-?B-modulated miR-19a-3p enhances malignancy of human ovarian cancer cells through inhibition of IGFBP-3 expression. Mol Carcinog 58:2254-2265 (2019). PubMed: 31513316
- Yao TH et al. Propranolol induces hemangioma endothelial cell apoptosis via a p53-BAX mediated pathway. Mol Med Rep 18:684-694 (2018). PubMed: 29767244
- Marthandan S et al. Conserved genes and pathways in primary human fibroblast strains undergoing replicative and radiation induced senescence. Biol Res 49:34 (2016). PubMed: 27464526
- Goyal R et al. Antenatal maternal long-term hypoxia: acclimatization responses with altered gene expression in ovine fetal carotid arteries. PLoS One 8:e82200 (2013). WB ; Sheep . PubMed: 24367503
- Yoo EG et al. Insulin-like growth factor-binding protein-3 mediates high glucose-induced apoptosis by increasing oxidative stress in proximal tubular epithelial cells. Endocrinology 152:3135-42 (2011). WB ; Pig . PubMed: 21652730