製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to IGFBP3
  • 特異性
    Ab36529 is specific to IGFBP3. It shows less than 0.05% reactivity with human IGFBPs 1, 2, 4, 5 or 6, as determined by EIA.
  • アプリケーション
    適用あり: ELISA, WB, Dot blotmore details
  • 種交差性
    交差種: Human
  • 免疫原

    IGFBP3 plus epitope of human IGFBP3.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab36529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
Dot blot
  • 追加情報
    Dot: 1/5000.
    ELISA: 1/8000.
    WB: A 1/5000 dilution detects 60ng IGFBP3. Predicted molecular weight: 32 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
    • 組織特異性
      Expressed by most tissues. Present in plasma.
    • 配列類似性
      Contains 1 IGFBP N-terminal domain.
      Contains 1 thyroglobulin type-1 domain.
    • 発生段階
      IGFBP3 levels are higher during extrauterine life and peak during puberty.
    • ドメイン
      The thyroglobulin type-1 domain mediates interaction with HN.
    • 翻訳後修飾
      Phosphorylation sites are present in the extracelllular medium.
    • 細胞内局在
      Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Acid stable subunit of the 140 K IGF complex antibody
      • Binding protein 29 antibody
      • Binding protein 53 antibody
      • BP 53 antibody
      • BP53 antibody
      • Growth hormone dependent binding protein antibody
      • IBP 3 antibody
      • IBP-3 antibody
      • IBP3 antibody
      • IBP3_HUMAN antibody
      • IGF binding protein 3 antibody
      • IGF-binding protein 3 antibody
      • IGFBP 3 antibody
      • IGFBP-3 antibody
      • IGFBP3 antibody
      • Insulin Like Growth Factor Binding Protein 3 antibody
      • Insulin-like growth factor binding protein 3 precursor antibody
      • Insulin-like growth factor-binding protein 3 antibody
      see all

    画像

    • ab36529 staining human fibroblast cells by ICC/IF.  Cells were fixed in formaldehyde, permeabilised in 0.5% triton for 5 min, and blocked with 4% BSA for 1 hour at 20°C.  The primary antibody was diluted 1/200 with 4% BSA/TBST and incubated for 2 hours at 20°C.  An Alexa Fluor® 594 conjugated donkey anti-mouse was used as the secondary antibody.

      Red fluorescence shows IGFBP3, blue is DNA.

      See Abreview

    プロトコール

    参考文献

    This product has been referenced in:
    • Chenau J  et al. The cell line secretome, a suitable tool for investigating proteins released in vivo by tumors: application to the study of p53-modulated proteins secreted in lung cancer cells. J Proteome Res 8:4579-91 (2009). WB ; Human . Read more (PubMed: 19639960) »
    • Heath KE  et al. Primary acid-labile subunit deficiency due to recessive IGFALS mutations results in postnatal growth deficit associated with low circulating insulin growth factor (IGF)-I, IGF binding protein-3 levels, and hyperinsulinemia. J Clin Endocrinol Metab 93:1616-24 (2008). Read more (PubMed: 18303074) »

    See all 2 Publications for this product

    レビューと Q&A

    I received some information regarding the immunogen of this product and thought that you might be interested in knowing. The synthetic peptide used as an immunogen for this antibody was derived from the central domain of IGFBP-3 and does not contai...

    Read More
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (fibroblast)
    Specification
    fibroblast
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.5% triton for 5 min
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C
    Username

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    投稿 Jan 09 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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