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Our Abpromise guarantee covers the use of ab109790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution) allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IGFBP3.|
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IGFBP3 (Biotin) (ab84228). Using ab84228 as the detection antibody and ab109790 as the capture antibody, the detection limit for recombinant Human IGFBP3 is 0.2 - 0.4 ng/well, using 100 µl/well antibody solution.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 32 kDa. The detection limit for recombinant Human IGFBP3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions|
ab109790 has not yet been referenced specifically in any publications.