The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
追加情報Is unsuitable for WB.
機能Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs.
組織特異性Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis.
配列類似性Belongs to the RRM IMP/VICKZ family. Contains 4 KH domains. Contains 2 RRM (RNA recognition motif) domains.
ドメインThe third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA.
細胞内局在Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa.
Detection of Human IGF2BP2 by Immunoprecipitation in 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab117827 at 6 µg/mg of lysate. For subsequent WB detection, another anti Human IGF2BP2 antibody was used. Detection: Chemiluminescence with exposure time of 30 seconds.
Anti-IGF2BP2 antibody (ab117827) 使用論文
has not yet been referenced specifically in any publications.