製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to IGF2
  • 特異性
    Mouse 20kDa IGF2 protein.Cross reacts with rat protein. Low cross reactivity was observed with human IGF2 protein.
  • アプリケーション
    適用あり: WB, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Mouse IGF2.
    Database link: P09535

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab63984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/200 - 1/2000. Predicted molecular weight: 20 kDa.
IHC-P 1/50 - 1/200.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
    Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
  • 関連疾患
    Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations.
  • 配列類似性
    Belongs to the insulin family.
  • 翻訳後修飾
    O-glycosylated with a core 1 or possibly core 8 glycan.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C11orf43 antibody
    • IGF 2 antibody
    • IGF II antibody
    • IGF-II antibody
    • IGF2 antibody
    • IGF2_HUMAN antibody
    • IGFII antibody
    • INSIGF antibody
    • insulin like growth factor 2 (somatomedin A) antibody
    • Insulin like Growth Factor 2 antibody
    • Insulin like growth factor II antibody
    • Insulin like growth factor II precursor antibody
    • Insulin like growth factor type 2 antibody
    • pp9974 antibody
    • Preptin antibody
    • putative insulin like growth factor II associated protein antibody
    • Somatomedin A antibody
    • Somatomedin-A antibody
    see all

画像

  • Immunohistochemical analysis of murine amygdala tissue, staining IGF2 with ab63984 at 1/200 dilution. An AlexaFluor®-conjugated anti-rabbit IgG was used as the secondary antibody.

参考文献

This product has been referenced in:
  • Jung S  et al. Decreased expression of extracellular matrix proteins and trophic factors in the amygdala complex of depressed mice after chronic immobilization stress. BMC Neurosci 13:58 (2012). IHC-Fr ; Mouse . Read more (PubMed: 22672618) »
  • Singh P  et al. Insulin-like growth factors are more effective than progastrin in reversing proapoptotic effects of curcumin: critical role of p38MAPK. Am J Physiol Gastrointest Liver Physiol 298:G551-62 (2010). Neutralising ; Rat . Read more (PubMed: 20133951) »

See all 2 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (brain)
Gel Running Conditions
Reduced Non-Denaturing (Native) (12% GEL)
Loading amount
30 µg
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Mar 08 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate bufer
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Suypriya Sen

Verified customer

投稿 May 13 2014

Abreviews
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Mouse Cell lysate - whole cell (Liver tissue)
Specification
Liver tissue
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Suypriya Sen

Verified customer

投稿 May 13 2014

Thank you for contacting us.

We unfortunately do not offer trial size packages you have to buy the full volume of antibody.

Please note that in case this antibody does not work in applications (WB, IHC-P and IHC-Fr) in species (mouse)...

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Thank you for contacting us.

I have checked the sequence homology (http://fasta.bioch.virginia.edu/fasta_www2/fasta_www.cgi) between Insulin (http://www.uniprot.org/uniprot/P01308) and IGF2 (http://www.uniprot.org/uniprot/P01344); though there...

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Thank you for contacting us and for your interest in our products.

I am sorry to confirm that as far as we are aware, ab63984 has never been tested with samples from rat. All tested species cross-reactivity information covered by the guarant...

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Thank you for contacting us. Please find the requested Certificate of Compliance attached. Please let me know if you need any additional information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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