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Synthetic phospho-peptide derived from human IGF1 receptor around the phosphorylation site of tyrosine 1161
Our Abpromise guarantee covers the use of ab39398 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-FoFr||1/100. PubMed: 18216249|
|ICC||Use at an assay dependent concentration.|
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/1000. Predicted molecular weight: 155 kDa.|
|IP||Use at an assay dependent concentration.|
|IHC-FrFl||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
Western blot analysis using IGF1 Receptor antibody (ab39675, Lane 1, 2 and 3) and IGF1 Receptor (phospho-Tyr1161) antibody (ab39398, Lane 4 and 5).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue sections labeling IGF1 Receptor (phospho Y1161) with ab39398 at 1/100 dilution. The tissue was fixed with formaldehyde; heat mediated antigen retrieval was performed using a citrate buffer pH 6.0. The tissue was blocked with 5% serum for 1 hour at 23°C followed by incubation with ab39398 at 1/100 for 1 hour at 23°C. An undiluted polyclonal goat anti-rabbit HRP conjugated secondary antibody was used.