Anti-IGF1 Receptor 抗体 [alphaIR3] (ab16890)


  • 製品名Anti-IGF1 Receptor antibody [alphaIR3]
    IGF1 Receptor 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [alphaIR3] to IGF1 Receptor
  • 特異性Recognizes the human IGF-I Receptor. IGF-I Receptor (Ab-1)immunoprecipitates the a and ß subunits of the IGF-I receptor. IGF-I receptor (ab16890) blocks IGF-I binding to its receptor and may bind weakly to the insulin receptor (see application references). It also inhibits the growth of MCF-7 cells in culture suggesting the IGF-I receptor may be involved in autocrine regulation of cell growth (see application references). This antibody is not recommended for Western blotting or paraffin sections.
  • アプリケーション適用あり: Flow Cyt, IP, Neutralising, ICC/IFmore details
    適用なし: IHC-P or WB
  • 種交差性
    交差種: Hamster, Human
  • 免疫原

    Immunogen used was partially purified receptor from human placenta.

  • ポジティブ・コントロール
    • HepG2 cells



Our Abpromise guarantee covers the use of ab16890 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/10 - 1/20. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
Neutralising Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 1 - 5 µg/ml.
  • 追加情報Is unsuitable for IHC-P or WB.
  • ターゲット情報

    • 機能Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K-driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particular activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R.
      When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.
    • 組織特異性Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Expressed in a variety of tissues. Overexpressed in tumors, including melanomas, cancers of the colon, pancreas prostate and kidney.
    • 関連疾患Insulin-like growth factor 1 resistance
    • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
      Contains 4 fibronectin type-III domains.
      Contains 1 protein kinase domain.
    • 翻訳後修飾Autophosphorylated on tyrosine residues in response to ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Autophosphorylation occurs in a sequential manner; Tyr-1165 is predominantly phosphorylated first, followed by phosphorylation of Tyr-1161 and Tyr-1166. While every single phosphorylation increases kinase activity, all three tyrosine residues in the kinase activation loop (Tyr-1165, Tyr-1161 and Tyr-1166) have to be phosphorylated for optimal activity. Can be autophosphorylated at additional tyrosine residues (in vitro). Autophosphorylated is followed by phosphorylation of juxtamembrane tyrosines and C-terminal serines. Phosphorylation of Tyr-980 is required for IRS1- and SHC1-binding. Phosphorylation of Ser-1278 by GSK-3beta restrains kinase activity and promotes cell surface expression, it requires a priming phosphorylation at Ser-1282. Dephosphorylated by PTPN1.
      Polyubiquitinated at Lys-1168 and Lys-1171 through both 'Lys-48' and 'Lys-29' linkages, promoting receptor endocytosis and subsequent degradation by the proteasome. Ubiquitination is facilitated by pre-existing phosphorylation.
      Sumoylated with SUMO1.
      Controlled by regulated intramembrane proteolysis (RIP). Undergoes metalloprotease-dependent constitutive ectodomain shedding to produce a membrane-anchored 52 kDa C-Terminal fragment which is further processed by presenilin gamma-secretase to yield an intracellular 50 kDa fragment.
    • 細胞内局在Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CD221 antibody
      • CD221 antigen antibody
      • IGF 1 receptor antibody
      • IGF 1R antibody
      • IGF I receptor antibody
      • IGF-I receptor antibody
      • Igf1r antibody
      • IGF1R_HUMAN antibody
      • IGFIR antibody
      • IGFIRC antibody
      • IGFR antibody
      • Insulin like growth factor 1 receptor antibody
      • Insulin like growth factor 1 receptor precursor antibody
      • Insulin-like growth factor 1 receptor beta chain antibody
      • Insulin-like growth factor I receptor antibody
      • JTK13 antibody
      • MGC142170 antibody
      • MGC142172 antibody
      • MGC18216 antibody
      • Soluble IGF1R variant 1 antibody
      • Soluble IGF1R variant 2 antibody
      see all

    Anti-IGF1 Receptor antibody [alphaIR3] 画像

    • ICC/IF image of ab16890 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16890, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Overlay histogram showing LoVo cells stained with ab16890 (red line). The cells were fixed with 4% paraformaldehyde (10 mins) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16890, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in LoVo cells fixed with methanol (5 min) used under the same conditions.

      Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    Anti-IGF1 Receptor antibody [alphaIR3] (ab16890) 使用論文

    This product has been referenced in:
    • O'Neill AF  et al. Targeted imaging of Ewing sarcoma in preclinical models using a 64Cu-labeled anti-CD99 antibody. Clin Cancer Res 20:678-87 (2014). Read more (PubMed: 24218512) »
    • Sirianni R  et al. Targeting estrogen receptor-a reduces adrenocortical cancer (ACC) cell growth in vitro and in vivo: potential therapeutic role of selective estrogen receptor modulators (SERMs) for ACC treatment. J Clin Endocrinol Metab 97:E2238-50 (2012). Read more (PubMed: 23074235) »

    See all 5 Publications for this product

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    Ich versuche grade, mehr Information bezüglich des Immunogens für ab16890,um einen Sequenzvergleich mit dem Mausprotein durchführen. Ich...

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    Vielen Dank für Ihre Anfrage.

    Der ab16890 Anti-IGF1 Receptor antibody [alphaIR3] sollte gut für Ihre Zwecke geeignet sein:

    Er wurde bereits im Funktional Assay getestet
    Er blockiert den Rezeptor (allerdings auch zu einem gewi...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Hamster Cell lysate - whole cell (CHO cells)
    Total protein in input 50 µg
    Specification CHO cells
    Immuno-precipitation step Protein A/G

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    Verified customer

    投稿 Sep 18 2007

    Thank you for your enquiry. This antibody recognizes the ~130 kDa a (alpha) and the ~90 kDa b (beta) subunits of IGF-I receptor. Please do not hesitate to contact us for further assistance.

    Thank you for your enquiry. Further to correspondence with my colleagues in the lab I can tell you that ab16890 is in fact reconstituted in phosphate buffered saline (PBS), pH 7.4. I have updated the datasheet to reflect this. I hope this inform...

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    Thank you for your enquiry. Free or trial sized samples are considered on a case by case basis. On this occasion unfortunately I cannot offer you a free sample. However, should you decide to go ahead and purchase this product, please let us know ho...

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    Thank you for your enquiry. Further to correspondence with my colleagues in the lab I can tell you that ab16890 is in fact reconstituted in phosphate buffered saline (PBS), pH 7.4. I hope this information helps, please do not hesitate to contact...

    Read More