製品の概要

  • 製品名Anti-IGF1 Receptor alpha subunit antibody [24-60]
    IGF1 Receptor alpha subunit 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [24-60] to IGF1 Receptor alpha subunit
  • 特異性Weakly reacts with Rabbit and Pig. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • アプリケーション適用あり: IP, Inhibition Assaymore details
    適用なし: WB
  • 種交差性
    交差種: Rabbit, Human, Pig
  • 免疫原

    IGF-1R/3T3 mouse fibroblasts transfected with human IGF1 Receptor alpha subunit cDNA.

  • エピトープaa 184-283
  • ポジティブ・コントロール
    • Placenta or breast carcinoma
  • 特記事項

    ab80548 acts as a weak IGF-like agonist in Bioassays (Thymidine incorporation into DNA).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 10mM PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein A purified
  • 一次抗体 備考ab80548 acts as a weak IGF-like agonist in Bioassays (Thymidine incorporation into DNA).
  • ポリ/モノモノクローナル
  • クローン名24-60
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab80548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at 2 µg/mg of lysate. Substantially (>80%) inhibits IGF-1 binding.
Inhibition Assay Use a concentration of 1.5 µg/ml.
  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 関連性IGF1 Receptor is a tetramer of 2 alpha and 2 beta chains, linked by disulfide bonds. IGF1 Receptor, like insulin receptor, is synthesized as a single polypeptide which is glycosylated and proteolytically cleaved to give the alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The alpha-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity. It is assumed that, as for the insulin receptor, the tyrosine kinase activity is essential for IGF-1 action. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chain carries the kinase domain. It interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. IGF1 Receptor forms a hybrid receptor with INSR, the hybrid being a tetramer consisting of 1 alpha chain and 1 beta chain of INSR and 1 alpha chain and 1 beta chain of IGF1R.
    • 細胞内局在Membrane; Single-pass type I membrane protein.
    • 参照データベース
    • 別名
      • CD221 antibody
      • CD221 antigen antibody
      • IGF I receptor antibody
      • IGFIR antibody
      • Insulin like growth factor I receptor antibody
      • JTK13 antibody
      see all

    Anti-IGF1 Receptor alpha subunit antibody [24-60] (ab80548) 使用論文

    This product has been referenced in:
    • Middleton J  et al. Insulin-like growth factor (IGF) receptor, IGF-I, interleukin-1 beta (IL-1 beta), and IL-6 mRNA expression in osteoarthritic and normal human cartilage. J Histochem Cytochem 44:133-41 (1996). Read more (PubMed: 8609369) »
    • Soos MA  et al. Immunological studies of type I IGF receptors and insulin receptors: characterisation of hybrid and atypical receptor subtypes. Adv Exp Med Biol 343:145-57 (1993). Read more (PubMed: 7514333) »

    See all 4 Publications for this product

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    There are currently no Abreviews or Questions for ab80548.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"