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Our Abpromise guarantee covers the use of ab83137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IGF1 (ab9572). Allows for the detection of at least 0.2 - 0.4 ng/well of recombinant IGF1.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-I is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|ELISA||Use a concentration of 0.25 - 0.1 µg/ml. Direct ELISA. Allows for the detection of at least 0.2 - 0.4 ng/well of recombinant IGF1.|
ab83137 has not yet been referenced specifically in any publications.