製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to IGF1
  • アプリケーション
    適用あり: ICC/IF, IHC-P, IHC (PFA fixed)more details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: a wide range of other species
  • 免疫原

    Synthetic human insulin-like growth factor 1 [Ac60-70] covalently attached onto a carrier protein.

  • ポジティブ・コントロール
    • This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: Hek293.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab40657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC (PFA fixed) 1/1000.

ターゲット情報

  • 機能
    The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.
  • 関連疾患
    Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
  • 配列類似性
    Belongs to the insulin family.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 製品の状態
    There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA.
  • 別名
    • IBP1 antibody
    • IGF I antibody
    • IGF IA antibody
    • IGF IB antibody
    • IGF-I antibody
    • Igf1 antibody
    • IGF1_HUMAN antibody
    • IGF1A antibody
    • IGFI antibody
    • IGFIA antibody
    • Insulin like growth factor 1 (somatomedin C) antibody
    • Insulin like growth factor 1 antibody
    • Insulin like growth factor IA antibody
    • Insulin like growth factor IB antibody
    • Insulin-like growth factor I antibody
    • Mechano growth factor antibody
    • MGF antibody
    • OTTHUMP00000195080 antibody
    • OTTHUMP00000195081 antibody
    • OTTHUMP00000195082 antibody
    • OTTHUMP00000195083 antibody
    • OTTHUMP00000195084 antibody
    • Somatomedia C antibody
    • Somatomedin C antibody
    • Somatomedin-C antibody
    see all

画像

  • ab40657 staining IGF1 in murine prostate tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed with paraformaldehyde and an antigen retrieval step was performed using citrate buffer. Samples were then blocked with 1% serum for 20 minutes at 25°C and then incubated with ab40657 at a 1/1000 dilution for 18 hours at 4°C. The secondary used was a biotin conjugated horse polyclonal used at a 1/50 dilution.

    See Abreview

  • ab40657 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab40657 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of rat colon tissue, staining IGF1 with ab40657.

    Tissue was fixed with formaldehyde and blocked with 1% blocking solution for 15 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/400 in BSA in TBS) for 1 hour. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:
  • Thomsen J  et al. PAPP-A proteolytic activity enhances IGF bioactivity in ascites from women with ovarian carcinoma. Oncotarget 6:32266-78 (2015). IHC . Read more (PubMed: 26336825) »
  • Dhakal P  et al. Neonatal Progesterone Programs Adult Uterine Responses to Progesterone and Susceptibility to Uterine Dysfunction. Endocrinology 156:3791-803 (2015). Read more (PubMed: 26204463) »

See all 3 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tooth)
Antigen retrieval step
None
Permeabilization
No
Specification
tooth
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 02 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (15%)
Sample
Mouse Cell lysate - whole cell (MC3T3-E1 osteoblasts)
Specification
MC3T3-E1 osteoblasts
Treatment
Pre and post differentiation with osteogenic medium
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Benny Garfinkel

Verified customer

投稿 Aug 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TE pH 9.0
Sample
Mouse Tissue sections (Ovary)
Specification
Ovary
Permeabilization
No
Fixative
Paraformaldehyde
Username

Mr. Benny Garfinkel

Verified customer

投稿 Aug 12 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Colon)
Specification
Colon
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6 Citrate
Permeabilization
No
Blocking step
DAKO PB as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Nov 30 2012

Thank you for contacting Abcam regarding ab40657.


The sequence of mature IGF -1 (Ac-60-70) is as follows:Ac-YCAPLKPAKSA


I apologize for any confusion created. I have requested that our online datasheet be updated.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Prostate)
Specification
Prostate
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Dr. Lucila Sackmann Sala

Verified customer

投稿 Apr 10 2012

Thank you for contacting us and for your Abreview.

I have linked the discount code to your Abreview. The code is now activated, you can place an order for a free antibody.

I hope this information is helpful to you. Please do not hes...

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Expiration date: JJ MM YYYY

I am very pleased to hear you would like to accept our offer and test ab40657 in Mouse (100% homology with the immunogen). This code will give you 1 freeprimary antibodybefore the expira...

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Thank you for your enquiry and your interest in our products. Native human IGF-1 is a 70 amino acid long peptide. We wanted to develop an antibody that bounds specifically to the -COOH terminus of the whole peptide, so we synthesized an 11-mer, i.e....

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Thank you for your enquiry. I am sorry to hear that you are having problems with using one of our products. This product is supplied as lyophilized powder, which should be stored at 4oC until rehydrated. Histochemical grade antibodies should be d...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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