製品の概要

  • 製品名Anti-IgE antibody [5D4]
    IgE 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [5D4] to IgE
  • 特異性Specific for IgE Ce2-domain specific
  • アプリケーション適用あり: ELISA, Sandwich ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length native protein (purified) corresponding to Human IgE. IgE from myelomatous human serum.

  • 特記事項Concentration varies from lot to lot and can be provided on request.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein A purified
  • 特記事項(精製)Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.
  • ポリ/モノモノクローナル
  • クローン名5D4
  • ミエローマSp2/0
  • アイソタイプIgG2a
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab7295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution. Ab7295 can be used for the detection of total IgE levels and IgE antibodies by ELISA
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [4F4] to IgE (HRP) (ab7294). Can be used as Capture antibody with recommended pair.

ターゲット情報

  • 関連性IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
  • 細胞内局在Cell Membrane
  • 参照データベース
  • 別名
    • Igh2 antibody
    • IGHE antibody
    • IGHEP1 antibody
    • Immunoglobulin heavy constant epsilon antibody

Anti-IgE antibody [5D4] 画像

  • Standard curve: Capture anitbody (ab7295) at 5 ug/ml in PBS, human IgE from 100 ng/ml to 1.56 ng/ml, detection antibody (ab7294, HRP conjugated) at 0.7 ug/ml concentration.

Anti-IgE antibody [5D4] (ab7295) 使用論文

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Thank you for contacting us.   I have received the following information from the lab: We are using ab7295 according to the datasheet recommendation as the capture antibody, not as detection antibody. The detection antibody ab7295 was used with the...

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Thank you for your enquiry. In our internal assay for anti-IgE MAb testing, highly purified myelomatous IgE(VL) was adsorbed on solid phase from solution (5 microgram/ml) in bicarbonate buffer pH 9.5. Ab7295 was applied for 60-120 min onto antige...

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Thank you for your enquiry and for completing our technical questionnaire. I am sorry to hear that you have been having difficulties with this antibody. I would greatly appreciate it if you could tell me of the concentration that you have been using...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"