The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 - 5 µg/ml. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
Flow Cytometry - Anti-IgE antibody [4H10] (FITC) (ab77239)This image is courtesy of an anonymous Abreview
ab77239 staining IgE in Human basophil granulocytes by Flow Cytometry. Cells were peripheral blood cells, separated by Ficoll density separation, followed by magnetic isolation. The sample was incubated with the primary antibody (3μg/ml in PBS with Human IgG (Fc-block)) for 45 minutes at 4°C.
Gervais FG et al. Pharmacological characterization of MK-7246, a potent and selective CRTH2 (chemoattractant receptor-homologous molecule expressed on T-helper type 2 cells) antagonist. Mol Pharmacol79:69-76 (2011).
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