Anti-IgA Secretory Component 抗体 [SC-05] (ab3924)

製品の概要

  • 製品名Anti-IgA Secretory Component antibody [SC-05]
    IgA Secretory Component 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [SC-05] to IgA Secretory Component
  • 特異性ab3924 reacts with 80 kDa human secretory component glycoprotein (both free and bound in secretory IgA). The recognized glycoprotein is a specific membrane marker of glandular carcinomas.
  • アプリケーション適用あり: IHC-Fr, IP, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length native protein (purified) corresponding to Human IgA Secretory Component. Affinity-purified secretory component from human colostrum.

  • ポジティブ・コントロール
    • Secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland.
  • 特記事項


    Examples of positive human tissues: secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度>95% by SDS-PAGE
  • 特記事項(精製)Purified from hybridoma culture supernatant by protein A affinity chromatography.
  • 一次抗体 備考Examples of positive human tissues: secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland.
  • ポリ/モノモノクローナル
  • クローン名SC-05
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 61 kDa). See Abreview. Use under non-reducing conditions.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 関連性IgA has two subclasses (IgA1 and IgA2) and can be produced as a monomeric as well as a dimeric form. The IgA dimeric form is the most prevalent and is also called secretory IgA (sIgA). The secretory component of sIgA protects the immunoglobulin from being degraded by proteolytic enzymes, thus sIgA can survive in the harsh gastrointestinal tract environment and provide protection against microbes that multiply in body secretions. sIgA can also inhibit inflammatory effects of other immunoglobulins. Secretory component is a proteolytic cleavage product of the polymeric immunoglobulin receptor which remains associated with dimeric IgA in sero-mucus secretions. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen. Secretory components wrap around two IgA units joined by a J chain protein fragment, resulting in a >--< configuration, with each of the two antigen binding regions of the two constituent y-shaped antibodies exposed. One identified function of secretory components is to protect IgA antibodies from degradation by the gastric acids and enzymes of the digestive system. This property is especially important in the transfer of immune system components during breastfeeding.

Anti-IgA Secretory Component antibody [SC-05] 画像

  • All lanes : Anti-IgA Secretory Component antibody [SC-05] (ab3924) at 1 µg/ml

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
    Secretory Component Glycoprotein contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • ab3924 staining IgA Secretory Component in human normal breast tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in methanol, blocked using 10% serum for 5 minutes at 25°C and then incubated with ab3924 at a 1/100 dilution for 1 hour at 25°C. The secondary used was an undiluted HRP conjugated goat polyclonal.The images show stainings of normal breast glands from two different patients. The antibody mostly stains the apical/luminal region of luminal epithelial cells, but the intensity varies from patient to patient.

    See Abreview

  • Immunohistochemical analysis of Human breast cancer tissue, staining IgA Secretory Component with ab3924.

    Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6). Tissue was blocked with 10% normal goat serum before incubating with primary antibody (1/50) for 2 hours at room temperature. Staining was primarily in the cytoplasm.

Anti-IgA Secretory Component antibody [SC-05] (ab3924) 使用論文

This product has been referenced in:
  • Wu Q  et al. Extracellular matrix protein 1 is correlated to carcinogenesis and lymphatic metastasis of human gastric cancer. World J Surg Oncol 12:132 (2014). IHC-P ; Human . Read more (PubMed: 24779890) »
  • Wu QW  et al. Expression and clinical significance of extracellular matrix protein 1 and vascular endothelial growth factor-C in lymphatic metastasis of human breast cancer. BMC Cancer 12:47 (2012). IHC-P ; Human . Read more (PubMed: 22284579) »

See all 6 Publications for this product

Product Wall

I have had a look at the nature of this protein: Extracellular matrix protein 1 (http://www.uniprot.org/uniprot/Q16610)

Unfortunately, I do not think it is scientifically to detect only ECM1b without detecting ECM1a.

The difference ...

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It appears that there is a nomenclature difference between your target of interest (secretory component) and our antibodies that are anti "secretory component glycoprotein" which is also referred to as "Extracellular matrix protein 1" and "secretory co...

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For ab3924, the links to the databases and target information mention "Extracellular matrix protein 1" which is an alternative name for "secretory component p85" and "secretory component glycoprotein" according to the uniprot file below:
http://www...

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Thank you for contacting us.

In general, the selection of the immunogen varies from antibody to antibody. Some antibodies are raised against synthetic peptides (based ona protein sequence which was derived originally from a DNA sequence), so...

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Thank you for taking the time to respond and for contacting Abcam.

With over 70,000 products it is logistically impossible for us to offer free samples. I hope you understand.

Please let me know if you have any questions.

Thank you for contacting Abcam.

I am sorry to hear that Ab55676 has not performed well for you. Although the peptide sequence has excellent identity with the mouse homolog, this product is not guaranteed to work in mouse tissues. High backgro...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Normal breast)
Specification Normal breast
Fixative Methanol
Blocking step Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
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Verified customer

投稿 Jan 04 2012

Thank you for contacting us. Unfortunately we do not have any specific information about the epitope of Anti-Secretory Component Glycoprotein antibody [SC-05] (ab3924). We do however know that the epitope recognised is reduction-resistant and prese...

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Thank you for your enquiry. Unfortunately the only information the originator of the antibody was able to provide was mostly on the datasheet: The antibody reacts with 80 kDa human secretory component glycoprotein (both free and bound in secretory ...

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Thank you for your enquiry. There is no information currently available about the working dilution for this antibody in IHC and Western blot. For a purified antibody, we recommend starting dilutions for Western blot to be 1 ug/mL and for IHC, 10 ug/mL....

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