Anti-IgA Affibody® Molecule (ab36058)
製品の概要
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製品名
Anti-IgA Affibody® Molecule -
アプリケーション
適用あり: ELISAmore details -
種交差性
交差種: Human -
免疫原
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特記事項
This product is a recombinant protein produced in E.coli.
What are Affibody Molecules?
Affibody® affinity ligands are unique research reagents, produced using innovative protein-engineering technologies. They are small, simple proteins composed of a three-helix bundle based on the scaffold of one of the IgG-binding domains of Protein A. Protein A is a surface protein from the bacterium Staphylococcus aureus. This scaffold has excellent features as an affinity ligand and can be designed to bind with high affinity to any given target protein. The domain consists of 58 amino acids, 13 of which are randomized to generate Affibody® libraries with a large number of ligand variants. Thus, the libraries consist of a multitude of protein ligands with an identical backbone and variable surface-binding properties. In function, Affibody® Molecules mimic monoclonal antibodies. Compared to antibodies, the most striking dissimilarity of Affibody® Molecules is the small size. Affibody® Molecules have a molecular weight of 6kDa, compared to the molecular weight of antibodies, which is 150kDa. In spite of its small size, the binding site of Affibody® Molecules is similar to that of an antibody. The advantages of Affibody® Molcules over antibodies are: -their small size -the simple structure of the molecules -its robust physical properties; able to withstand a broad range of analytical conditions, including extreme pH and elevated temperature -its ability to fold correctly intracellularly -the fast and cost effective production in bacteria -the potential to couple Affibody® Molecules in multimeric constructs Affibody® Molecules have highly competitive properties for applications within affinity purification, sample preparation, protein detection and in vitro diagnostics.This Anti-Fibrinogen Affibody® Molecule is modified with a unique C-terminal cysteine for directed single-point chemical modification, facilitating coupling to matrices. However, tail-to-tail dimers are spontaneously generated via a disulphide bridge between the C-terminal cysteines. Prior to coupling via the C-terminal the Affibody® Molecule needs to be reduced to expose the reactive cysteine residue. Recommended reducing condition is 20mM DTT at a pH above 7.5 and incubation at room temperature for 2 hours. Remove excess DTT by passage through a desalting column, not by dialysis. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
THIS AFFIBODY® MOLECULE REQUIRES CONJUGATION TO A SUITABLE LABEL BEFORE USE. PLEASE REFER TO THE "PROTOCOLS" LINK BELOW.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Constituents: 0.079% Ammonium bicarbonate, PBS
No preservative -
Concentration information loading...
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特記事項(精製)
The purity of this product is >98% as determined by SDS-PAGE and RP-HPLC analysis. -
研究分野
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関連性
Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. -
細胞内局在
Secreted -
別名
- FLJ14473
- FLJ35065
- FLJ35500
see all -
参照データベース
- Entrez Gene: 3493 Human
- Entrez Gene: 3494 Human
- Omim: 146900 Human
- Omim: 147000 Human
- SwissProt: P01876 Human
- SwissProt: P01877 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab36058の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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AP |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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特記事項 |
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AP
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
画像
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Results quantitative ELISA
The Anti-IgA Affibody® molecule can be used as capture reagent in a sandwich ELISA in combination with a goat anti-IgA antibody as the detection reagent. Titration of IgA gives a sigmoid curve with a sensitivity of 0.2 ng IgA/ml (defined as two times background value).
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Analysis of iga concentration in depleted serum
The remaining IgA in samples from serum, depleted from IgA by passage through an Anti-IgA Affibody® molecule coupled column, was analyzed using the Anti-IgA Affibody® ELISA. Concentration of IgA in flow through samples from 60 ul, 0.2 ml and 0.4 ml depleted plasma was analyzed and the percentage of achieved depletion was calculated. The data is presented in table 1.
Analysis of iga concentration in depleted serum
The remaining IgA in samples from serum, depleted from IgA by passage through an Anti-IgA Affibody® molecule coupled column, was analyzed using the Anti-IgA Affibody® ELISA. Concentration of IgA in flow through samples from 60 ul, 0.2 ml and 0.4 ml depleted plasma was analyzed and the percentage of achieved depletion was calculated. The data is presented in table 1.
データシートおよび資料
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SDS download
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Datasheet download
プロトコール
参考文献 (0)
ab36058 は論文での使用が確認できていません。