製品の概要

  • 製品名
    Anti-IFT43 antibody
  • 製品の詳細
    Rabbit polyclonal to IFT43
  • アプリケーション
    適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 107-202 (EEVQEEDFVL QVAAPPSIQI KRVMTYRDLD NDLMKYSAIQ TLDGEIDLKL LTKVLAPEHE VREDDVGWDW DHLFTEVSSE VLTEWDPLQT EKEDPA) of Human IFT43 (Q96FT9).

  • ポジティブ・コントロール
    • IFT43-transfected HEK293T lysate; Human testis tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab121195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

ターゲット情報

  • 機能
    Component of IFT complex A (IFT-A) involved in retrograde ciliary transport along microtubules from the ciliary tip to the base.
  • 関連疾患
    Defects in IFT43 are a cause of cranioectodermal dysplasia type 1 (CED1) [MIM:218330]. CED1 is a disorder characterized by craniofacial, skeletal and ectodermal abnormalities. Clinical features include dolichocephaly (with or without sagittal suture synostosis), scaphocephaly, short stature, limb shortening, short ribs, narrow chest, brachydactyly, renal failure and hepatic fibrosis, small and abnormally shaped teeth, sparse hair, skin laxity and abnormal nails.
  • 配列類似性
    Belongs to the IFT43 family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C14orf179 antibody
    • ift43 antibody
    • IFT43_HUMAN antibody
    • Intraflagellar transport protein 43 homolog antibody

画像

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoskeleton. Recommended concentration of ab121195 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-IFT43 antibody (ab121195) at 1/250 dilution

    Lane 1 : Vector only transfected HEK293T lysate
    Lane 2 : IFT43-transfected HEK293T lysate (Co-expressed with a C-terminal myc-DDK tag ~3.1 kDa)

    Developed using the ECL technique
  • ab121195 at 1/50 dilution staining IFT43 in Paraffin-embedded Human testis tissue by Immunohistochemistry.

参考文献

ab121195 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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