Anti-IFT43 抗体 (ab121195)
Key features and details
- Rabbit polyclonal to IFT43
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-IFT43 antibody -
製品の詳細
Rabbit polyclonal to IFT43 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment, corresponding to amino acids 107-202 (EEVQEEDFVL QVAAPPSIQI KRVMTYRDLD NDLMKYSAIQ TLDGEIDLKL LTKVLAPEHE VREDDVGWDW DHLFTEVSSE VLTEWDPLQT EKEDPA) of Human IFT43 (Q96FT9).
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ポジティブ・コントロール
- IFT43-transfected HEK293T lysate; Human testis tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121195の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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特記事項 |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ターゲット情報
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機能
Component of IFT complex A (IFT-A) involved in retrograde ciliary transport along microtubules from the ciliary tip to the base. -
関連疾患
Defects in IFT43 are a cause of cranioectodermal dysplasia type 1 (CED1) [MIM:218330]. CED1 is a disorder characterized by craniofacial, skeletal and ectodermal abnormalities. Clinical features include dolichocephaly (with or without sagittal suture synostosis), scaphocephaly, short stature, limb shortening, short ribs, narrow chest, brachydactyly, renal failure and hepatic fibrosis, small and abnormally shaped teeth, sparse hair, skin laxity and abnormal nails. -
配列類似性
Belongs to the IFT43 family. - Information by UniProt
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参照データベース
- Entrez Gene: 112752 Human
- Omim: 614068 Human
- SwissProt: Q96FT9 Human
- Unigene: 532626 Human
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別名
- C14orf179 antibody
- ift43 antibody
- IFT43_HUMAN antibody
- Intraflagellar transport protein 43 homolog antibody
画像
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Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoskeleton. Recommended concentration of ab121195 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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All lanes : Anti-IFT43 antibody (ab121195) at 1/250 dilution
Lane 1 : Vector only transfected HEK293T lysate
Lane 2 : IFT43-transfected HEK293T lysate (Co-expressed with a C-terminal myc-DDK tag ~3.1 kDa)
Developed using the ECL technique. -
ab121195 at 1/50 dilution staining IFT43 in Paraffin-embedded Human testis tissue by Immunohistochemistry.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab121195 は論文での使用が確認できていません。