製品の概要

  • 製品名Anti-IFT20 antibody
    IFT20 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to IFT20
  • 特異性Reactive against mammalian transfected lysate.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    IFT20 (NP_777547, 1 a.a. ~ 148 a.a) full-length human protein.

  • ポジティブ・コントロール
    • Transfected 293T golgi apparatus lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab73110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: 1/500 - 1/1000. Detects a band of approximately 16 kDa.

    Detection antibody.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性Eukaryotic cilia are assembled via intraflagellar transport (IFT) in which large protein particles are motored along ciliary microtubules. The IFT20 subunit of a particle is localized to the Golgi complex, plus the basal body and cilia. In living cells, fluorescently tagged IFT20 is highly dynamic and moves between the Golgi complex and the cilium as well as along ciliary microtubules. It has been suggested that IFT20 functions in the delivery of ciliary membrane proteins from the Golgi complex to the cilium. There are three different isoforms.
    • 細胞内局在Golgi apparatus. Cilium. Centrosome, centriole.
    • 参照データベース
    • 別名
      • hIFT20 antibody
      • Intraflagellar transport 20 homolog (Chlamydomonas) antibody
      • Intraflagellar transport protein 20 like antibody
      • Intraflagellar transport protein IFT20 antibody

    Anti-IFT20 antibody 画像

    • All lanes : Anti-IFT20 antibody (ab73110) at 1/500 dilution

      Lane 1 : IFT20 transfected 293T cell line
      Lane 2 : Non transfected 293T cell line

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Observed band size : 16 kDa (why is the actual band size different from the predicted?)

    Anti-IFT20 antibody (ab73110) 使用論文

    This product has been referenced in:
    • Mandell MA  et al. TRIM17 contributes to autophagy of midbodies while actively sparing other targets from degradation. J Cell Sci 129:3562-3573 (2016). Read more (PubMed: 27562068) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab73110.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"