製品の概要
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製品名Anti-IFT20 antibody
IFT20 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to IFT20
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由来種Rabbit
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アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
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種交差性交差種: Mouse, Human
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免疫原
Synthetic peptide corresponding to a region within internal sequence amino acids 50-80 of Human IFT20, conjugated to KLH.
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ポジティブ･コントロール
- Mouse lung tissue lysates; Human hepatocarcinoma tissue This antibody gave a positive result when used in the following methanol fixed cell lines: WI 38
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
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バッファーPreservative: 0.09% Sodium Azide
Constituents: PBS -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）ab103693 is purified through a protein A column, followed by peptide affinity purification.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab103693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/100 - 1/500. Predicted molecular weight: 15 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 5 µg/ml. |
ターゲット情報
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関連性Eukaryotic cilia are assembled via intraflagellar transport (IFT) in which large protein particles are motored along ciliary microtubules. The IFT20 subunit of a particle is localized to the Golgi complex, plus the basal body and cilia. In living cells, fluorescently tagged IFT20 is highly dynamic and moves between the Golgi complex and the cilium as well as along ciliary microtubules. It has been suggested that IFT20 functions in the delivery of ciliary membrane proteins from the Golgi complex to the cilium. There are three different isoforms.
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細胞内局在Golgi apparatus. Cilium. Centrosome, centriole.
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参照データベース
- Entrez Gene: 90410 Human
- Entrez Gene: 55978 Mouse
- SwissProt: Q8IY31 Human
- SwissProt: Q61025 Mouse
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別名
- hIFT20 antibody
- Intraflagellar transport 20 homolog (Chlamydomonas) antibody
- Intraflagellar transport protein 20 like antibody
- Intraflagellar transport protein IFT20 antibody
画像
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Immunocytochemistry/ Immunofluorescence - Anti-IFT20 antibody (ab103693)ab103693 stained WI 38 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103693 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - IFT20 antibody (ab103693)Anti-IFT20 antibody (ab103693) at 1/100 dilution + Mouse lung tissue lysates at 35 µg
Predicted band size: 15 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IFT20 antibody (ab103693)ab103693, at a 1/50 dilution, staining IFT20 in formalin fixed, paraffin embedded Human hepatocarcinoma tissue by Immunohistochemistry, followed by peroxidase conjugated secondary antibody and DAB staining.
プロトコール
参考文献
ab103693 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"