Anti-IFNGR1 (phospho Y457) 抗体 (ab61062)
Key features and details
- Rabbit polyclonal to IFNGR1 (phospho Y457)
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
製品の概要
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製品名
Anti-IFNGR1 (phospho Y457) antibody
IFNGR1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IFNGR1 (phospho Y457) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human, African green monkey
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide corresponding to Human IFNGR1 aa 400-500 (phospho Y457).
Database link: P15260 -
ポジティブ・コントロール
- Human brain tissue, COS7 cell extract.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab61062 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab61062の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 54 kDa).
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IHC-P |
1/50 - 1/100.
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 54 kDa). |
IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
Receptor for interferon gamma. Two receptors bind one interferon gamma dimer. -
関連疾患
Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance. -
配列類似性
Belongs to the type II cytokine receptor family.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
翻訳後修飾
Phosphorylated at Ser/Thr residues. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3459 Human
- Entrez Gene: 15979 Mouse
- Omim: 107470 Human
- SwissProt: P15260 Human
- SwissProt: P15261 Mouse
- Unigene: 520414 Human
- Unigene: 549 Mouse
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別名
- Antiviral Protein Type II antibody
- Antiviral protein, type 2 antibody
- AVP type II antibody
see all
画像
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Immunohistochemistry analysis of paraffin-embedded human brain tissue using IFNGR1 (phospho Y457) antibody (ab61062) at 1/50 - 1/100 dilution, in the presence (right panel) or absence (left panel) of phosphopeptide.
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All lanes : Anti-IFNGR1 (phospho Y457) antibody (ab61062) at 1/500 dilution
Lane 1 : COS7 cell extract with no phosphopeptide
Lane 2 : COS7 cell extract with phosphopeptide
Predicted band size: 54 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab61062 は 1 報の論文で使用されています。
- Shi J et al. JAK2 V617F stimulates proliferation of erythropoietin-dependent erythroid progenitors and delays their differentiation by activating Stat1 and other nonerythroid signaling pathways. Exp Hematol 44:1044-1058.e5 (2016). PubMed: 27473563