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Purified recombinant human interferon-alpha/beta receptor 1 (IFNAR1) extracellular domain expressed in insect Sf cells.
IFNAR1 is a member of the cytokine receptor superfamily which also includes receptors for interleukins, IFN gamma, ciliary neurotrophic factor, somatotrophin, erythropoietin, nerve growth factor, tumor necrosis factor, leukemia inhibitory factor, and oncostatin M. Some members of the family have an alpha chain with either low or high ligand binding affinity and at least one beta chain involved in signal transduction with either relatively low or no ligand binding affinity. Type I interferons, alpha and beta, induce a variety of effects on target cells including antiviral, antiproliferative, and immunomodulatory activities. The alpha and beta interferons compete to bind to a common cell surface receptor, while IFN gamma binds to a distinct receptor. IFNAR1 is very responsive to type I interferons and bind to IFN beta and IFN alpha subtypes. It is also functionally involved in signal transduction because of its association with the cytoplasmic tyrosine kinase JAK1. The type I interferons, alpha and beta, are produced by leukocytes (alpha subunits), fibroblasts (beta subtypes), lymphocytes (omega subtypes), and ruminant embryos (tau subtypes). Interferon receptors are generally found on most human cell types whatever their origin, even on cells poorly responsive to interferon. IFNAR1 is expressed on the cell surface in a variety of human cell lines.
Our Abpromise guarantee covers the use of ab10739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 3-10µl for 106 cells.|
|ELISA||Use a concentration of 0.5 - 1 µg/ml. The detection limit for recombinant human IFNAR1 is approximately 0.3 ng/well.|
|Neutralising||Use a concentration of 15 - 30 µg/ml. This will block 50 % of the binding of recombinant human IFN beta (20 ng/ml) to immobilized recombinant human IFNAR1 (1 µg/ml of a 100 µl solution coated in each well) in a functional ELISA.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 135 kDa.
The detection limit for recombinant human IFN-µ/ß R1 is ∼25 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively.