Anti-IFN gamma Receptor beta 抗体 [MMHGR-2] (ab25448)

製品の概要

  • 製品名Anti-IFN gamma Receptor beta antibody [MMHGR-2]
    IFN gamma Receptor beta 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MMHGR-2] to IFN gamma Receptor beta
  • 特異性ab25448 binds to but does not neutralise human interferon gamma receptor.
  • アプリケーション適用あり: WB, IP, Flow Cytmore details
    適用なし: Neut
  • 種交差性
    交差種: Human
  • 免疫原

    Human interferon gamma receptor beta.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab25448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 38 kDa.
IP Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
  • 追加情報Is unsuitable for Neut.
  • ターゲット情報

    • 機能Part of the receptor for interferon gamma. Required for signal transduction. This accessory factor is an integral part of the IFN-gamma signal transduction pathway and is likely to interact with GAF, JAK1, and/or JAK2.
    • 関連疾患Defects in IFNGR2 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
    • 配列類似性Belongs to the type II cytokine receptor family.
      Contains 2 fibronectin type-III domains.
    • 細胞内局在Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AF 1 antibody
      • AF-1 antibody
      • AF1 antibody
      • IFGR 2 antibody
      • IFGR2 antibody
      • IFN-gamma receptor 2 antibody
      • IFN-gamma-R2 antibody
      • IFNGR 2 antibody
      • IFNGR2 antibody
      • IFNGT 1 antibody
      • IFNGT1 antibody
      • IMD28 antibody
      • INGR2_HUMAN antibody
      • Interferon gamma receptor 2 (interferon gamma transducer 1) antibody
      • Interferon gamma receptor 2 antibody
      • Interferon gamma receptor accessory factor 1 antibody
      • Interferon gamma receptor beta chain antibody
      • Interferon gamma receptor chain 2 antibody
      • Interferon gamma transducer 1 antibody
      see all

    Anti-IFN gamma Receptor beta antibody [MMHGR-2] 画像

    • Overlay histogram showing Ramos cells stained with ab25448 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab25448, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    Anti-IFN gamma Receptor beta antibody [MMHGR-2] (ab25448) 使用論文

    This product has been referenced in:
    • Respa A  et al. Association of IFN-{gamma} Signal Transduction Defects with Impaired HLA Class I Antigen Processing in Melanoma Cell Lines. Clin Cancer Res 17:2668-2678 (2011). Flow Cyt ; Human . Read more (PubMed: 21248298) »

    See 1 Publication for this product

    Product Wall

    Please find attached list of anti IFN gamma antibodies. It seems ab8095 or ab8096 are the best match as per your requirements.

    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (Glial cell culture)
    Specification Glial cell culture
    Fixative Paraformaldehyde
    Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 5%
    Username

    Dr. Randal Moldrich

    Verified customer

    投稿 Jun 08 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"