Anti-IFI16 抗体 [2E3] (ab55328)
Key features and details
- Mouse monoclonal [2E3] to IFI16
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, Purification
- Reacts with: Human
- Isotype: IgG2b
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-IFI16 antibody [2E3]
IFI16 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2E3] to IFI16 -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-P, ICC/IF, Flow Cyt, Purificationmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment: FVNGVFEVHK KNVRGEFTYY EIQDNTGKME VVVHGRLTTI NCEEGDKLKL TCFELAPKSG NTGELRSVIH SHIKVIKTRK NKKDILNPDS SMETSPDFFF , corresponding to amino acids 630-730 of Human IFI16
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特記事項
This product was changed from ascites to tissue culture supernatant on 24/1/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.4 -
Concentration information loading...
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精製度
Tissue culture supernatant -
特記事項(精製)
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
2E3 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab55328の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 88 kDa.
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF | (2) |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
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Purification |
Use at an assay dependent concentration. PubMed: 22691496
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 88 kDa. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
Purification
Use at an assay dependent concentration. PubMed: 22691496 |
ターゲット情報
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関連性
Interferon (IFN) Inducible 16 (IFI16) protein belongs to a family of HIN 200 human and mouse proteins. IFI16 is a nuclear protein comtaining regulatory domains such as DNA binding domain, transcriptional regulatory domain and DAPIN/PAAD domain. IFI16 has three isotypes A, B, and C (85-95 kDa), which arise as a result of mRNA alternative splicing. All are phosphorylated on serine and threonine residues and can homo and heterodimerize. Expression is restricted to the nuclei of hematopoietic cells, fibroblasts and epithelial cells. IFI16 expression in hematopoietic cells of myeloid lineage is tightly regulated and highly induced in the differentiation and proliferation of the cell. Due to its localization in the nucleus, regulation of protein expression, and ability to bind DNA, it is assumed that IFI16 has a role in transcription regulation of cell differentiation. In addition, it was found that IFI16 can act as a transcriptional repressor and is involved in regulation and activation of p53 in cancer cells. -
細胞内局在
Nucleus. Cytoplasm. Note: Cellular distribution is dependent on the acetylation status of the multipartite nuclear localization signal (NLS); NLS acetylation promotes cytoplasmic localization. -
参照データベース
- Entrez Gene: 3428 Human
- Omim: 147586 Human
- SwissProt: Q16666 Human
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別名
- IFI16 antibody
- Gamma interferon inducible protein 16 antibody
- Gamma-interferon-inducible protein Ifi16 antibody
see all
画像
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All lanes : Anti-IFI16 antibody [2E3] (ab55328) at 1/1000 dilution
Lane 1 : Human sjsa1 whole cell lysate without siRNA to IFI16
Lane 2 : Human sjsa1 whole cell lysate with siRNA to IFI16
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : IRDye® 680-conjugated Mouse anti-mouse IgG polyclonal at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 88 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutesThis image was generated using the ascites version of the product.
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IFI16 antibody (ab55328) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.
This image was generated using the ascites version of the product.
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IFI16 antibody (ab55328) at 1μg/lane + HeLa cell lysate at 25μg/lane.
This image was generated using the ascites version of the product.
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Overlay histogram showing Jurkat cells stained with ab55328 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55328, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This image was generated using the ascites version of the product.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (22)
ab55328 は 22 報の論文で使用されています。
- Wang Z et al. Identification of a Metastasis-Related Protein IFI16 in Esophageal Cancer using a Proteomic Approach. J Cancer 13:1630-1639 (2022). PubMed: 35371314
- Liu L et al. IFI16 induces inflammation in hepatitis B virus-associated glomerulonephritis by regulating the Caspase-1/ IL-1 ß pathway. Diagn Pathol 17:39 (2022). PubMed: 35459254
- Mertens ME & Knipe DM Herpes Simplex Virus 1 Manipulates Host Cell Antiviral and Proviral DNA Damage Responses. mBio 12:N/A (2021). PubMed: 33563816
- Krump NA et al. Merkel Cell Polyomavirus Infection Induces an Antiviral Innate Immune Response in Human Dermal Fibroblasts. J Virol 95:e0221120 (2021). PubMed: 33883226
- Peng T et al. Tissue-Resident-Memory CD8+ T Cells Bridge Innate Immune Responses in Neighboring Epithelial Cells to Control Human Genital Herpes. Front Immunol 12:735643 (2021). PubMed: 34552595