製品の概要

  • 製品名Anti-IF3EI antibody
    IF3EI 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IF3EI
  • アプリケーション適用あり: IHC-P, ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide derived from N-terminal of human IF3EI.

  • ポジティブ・コントロール
    • HUVEC cells

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab64900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).

ターゲット情報

  • 機能Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
  • 配列類似性Belongs to the eIF-3 subunit L family.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • eIEF associated protein HSPC021 antibody
    • EIF3EIP antibody
    • eif3l antibody
    • EIF3L_HUMAN antibody
    • EIF3S11 antibody
    • EIF3S6IP antibody
    • Eukaryotic translation initiation factor 3 subunit 6 interacting antibody
    • Eukaryotic translation initiation factor 3 subunit 6-interacting protein antibody
    • Eukaryotic translation initiation factor 3 subunit E-interacting protein antibody
    • Eukaryotic translation initiation factor 3 subunit L antibody
    • eukaryotic translation initiation factor 3, subunit E interacting antibody
    • HSPC021 antibody
    • HSPC025 antibody
    • MSTP005 antibody
    see all

Anti-IF3EI antibody 画像

  • All lanes : Anti-IF3EI antibody (ab64900) at 1/500 dilution

    Lane 1 : extracts from HUVEC cells
    Lane 2 : extracts from HUVEC cells with immunizing peptide at 10 µg

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 67 kDa
    Observed band size : 67 kDa
  • IHC-P image of IF3EI antibody (ab64900) on pancreas tissue sections. The sections were fixed in Paraformaldehyde and underwent heat mediated antigen retrieval using Novacastra (pH6). The sections were then blocked in 3% H2O2 solution for 10 minutes at 22°C.

    See Abreview

Anti-IF3EI antibody (ab64900) 使用論文

ab64900 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample African Green Monkey Cell lysate - whole cell (Vero cells)
Loading amount 120000 cells
Specification Vero cells
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jun 27 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Pancreas)
Specification Pancreas
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Novocastra pH 6 retrieval buffer
Permeabilization No
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Dr. Aamir Ahmed

Verified customer

投稿 Jan 25 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"