製品の概要

  • 製品名Anti-IF-2(Mt) antibody
    IF-2(Mt) 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IF-2(Mt)
  • アプリケーション適用あり: IHC-P, ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment corresponding to amino acids 497-646 of Human IF-2(Mt)

  • ポジティブ・コントロール
    • Human cell line A431, Human plasma lysate, Human nasopharynx tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab121243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following is recommended: cell fixation with ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permealization with 0.1% Triton X-100 in PBS for 3x5 minutes.
WB 1/250 - 1/500.

ターゲット情報

  • 機能One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.
  • 組織特異性Expressed in all tissues examined. Highest level in skeletal muscle.
  • 配列類似性Belongs to the IF-2 family.
  • 細胞内局在Mitochondrion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • IF-2(Mt) antibody
    • IF-2Mt antibody
    • IF2(mt) antibody
    • IF2M_HUMAN antibody
    • mitochondrial antibody
    • mitochondrial translational initiation factor 2 antibody
    • Mtif2 antibody
    • Translation initiation factor IF-2 antibody
    • Translation initiation factor IF-2, mitochondrial antibody
    see all

Anti-IF-2(Mt) antibody 画像

  • All lanes : Anti-IF-2(Mt) antibody (ab121243) at 1/250 dilution

    Lane 1 : Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
    Lane 2 : RT-4 cell lysate
    Lane 3 : U-251 MG cell lysate
    Lane 4 : Human Plasma lysate
    Lane 5 : Human liver lysate
    Lane 6 : Human tonsil lysate
  • Immunohistochemical staining of paraffin embedded Human nasopharynx tissue, using 1/25 ab121243, shows strong membranous positivity in respiratory epithelial cells.
  • Immunofluorescent staining, using 4 µg/ml ab121243, in Human cell line A431 shows positivity in mitochondria. Antibody staining is shown in green.

Anti-IF-2(Mt) antibody (ab121243) 使用論文

ab121243 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"